摘要
【目的】为了鉴定控制干旱胁迫下绿豆根长生长基因。【方法】以绿豆RIL群体为研究材料,测定甘露醇溶液模拟干旱胁迫下绿豆幼苗根长的变化,并对正常条件下根长、处理条件下根长和相对根长性状进行定位。【结果】对照条件下根长、处理条件下根长和相对根长性状表型均为正态分布,说明根长性状是数量性状,受多基因控制;共检测到6个与根长相关QTLs,表型贡献率在5.94%~12.30%之间,其中对照根长的2个QTLs(qCRL4和qCRL5)位于第4和5连锁群、处理根长的2个QTLs(qSRL4和qSRL11)位于第4和11连锁群、相对根长的2个QTLs(qRRL6和qRRL11)位于第6和11连锁群;处理根长和相对根长性状的一个QTL重合于11号连锁群的Mchr10-27~Mchr10-47标记之间,对照根长和处理根长性状的一个QTL重合于4号连锁群的Mchr4-71~Mchr4-99标记之间,说明在这两个区间可能存在决定绿豆抗旱性的通用基因;检测到9对呈显著水平的互作影响,单个互作贡献率在23.05%~35.05%之间,且互作位点发生在非连锁标记之间。【结论】共分离出4个决定干旱胁迫下绿豆根长性状基因位点,研究结果为进一步克隆抗旱相关基因奠定基础。
【Objective】To identifying genes control root length of mung bean under drought stress.【Method】The root length of mung bean seedlings of RIL population under drought stress simulated by mannitol solution was measured.The putative QTLs of the root length of control condition(CRL),the root length of drought stress condition(SRL)and the relative root length(RRL)were analyzed.【Result】The phenotypes of the three traits are all normal distribution,indicating that these traits are quantitative characters,which are controlled by polygenes genetically.Six root length QTLs loci were detected in 4 chromosomes,which contribute the 5.94%-12.30%of the phenotype variation.Two CRL QTLs(qCRL4,qCRL5)were detected on the linkage group 4 and 5,two SRL QTLs(qSRL4,qSRL11)were detected on the linkage group 4 and 11,and two RRL QTLs(qRRL6,qRRL11)were detected on the linkage group 6 and 11.qCRL11 and qRRL11 are located in Mchr10-27/Mchr10-47 of the linkage group 11,and qCRL4 and qSRL4 are located in Mchr4-71/Mchr4-99 of the linkage group 4,indicating the universal drought-resistant gene of mung bean exist in the two intervals.Nine pair significant epistatic loci of SRL and RRL between non-linked marker sites distributing in 7 linkage groups were detected,and the contribution rate of a single interaction site was 23.05%-35.05%.【Conclusion】Four QTLs for root length were identified in mung bean under drought stress condition,which contribute to the mung bean root length gene cloning in the future.
作者
陈吉宝
CHEN Jibao(Nanyang Normal University,Collaborative Innovation Center of Water Security forWater Source Region of Mid-line of South-to-North Diversion Project of Henan Province,Nanyang 473061,He'nan,China)
出处
《四川农业大学学报》
CSCD
北大核心
2020年第4期416-422,共7页
Journal of Sichuan Agricultural University
基金
河南省高等学校重点科研项目(20B210015)。
关键词
绿豆
根长
QTL定位
干旱胁迫
mung bean
root length
QTL mapping
drought stress
