摘要
目的:比较女性类风湿关节炎(RA)患者miR-146a、miR-23b的表达水平与健康者的差异,分析miRNAs与炎症因子IL-17及实验室指标的相关性,以明确miR-146a、miR-23b是否与RA相关;分析雌激素对miR-146a、miR-23b及IL-17表达的影响,以明确雌激素影响RA的可能机制。方法:实时荧光定量PCR检测27例女性RA患者及27名女性健康体检者PBMCs中miR-146a、miR-23b的表达水平,酶联免疫吸附实验(ELISA)检测血浆IL-17的浓度,统计分析RA患者miR-146a、miR-23b及IL-17的表达水平与健康者的差异;收集RA患者临床实验室指标ESR、CRP、抗CCP抗体、RF的检测结果,计算DAS28评分,分析miR-146a、miR-23b分别与血浆IL-17、ESR、CRP、抗CCP抗体、RF及DAS28的相关性。再用雌二醇处理RA组和对照组的PBMCs后同样检测miR-146a、miR-23b及培养上清IL-17的水平,分析雌激素处理前后其水平变化。结果:女性RA患者PBMCs中miR-146a、miR-23b显著高于健康对照(P<0.05),miR-146a、miR-23b的表达水平分别与血浆IL-17、ESR、血清CRP、抗CCP抗体、RF、DAS28之间均无显著相关性(P>0.05)。经雌二醇处理后,女性RA患者PBMCs中miR-146a、miR-23b无显著变化(P>0.05),培养上清IL-17浓度显著增高(P<0.05),健康体检者PBMCs中miR-146a的表达水平明显下调(P<0.05),miR-23b的表达水平无明显变化(P>0.05)。结论:女性RA患者PBMCs中miR-146a、miR-23b的表达水平显著上调。雌激素可能一方面通过增强IL-17的表达参与RA的病程,另一方面通过下调PBMCs中miR-146a的表达阻止健康人向RA发展。
Objective:To compare the expression levels of miR-146a and miR-23b in female patients with rheumatoid arthritis(RA)and healthy people,and analyze the correlation between miRNAs and inflammatory factors IL-17 and laboratory indicators,so as to clarify whether miR-146a and miR-23b are related to RA,to analyze the effect of estrogen on the expression of miR-146a,miR-23b and IL-17,so as to clarify the possible mechanism of estrogen affecting RA.Methods:The expression levels of miR-146a and miR-23b were measured by RT-qPCR in 27 female RA patients and 27 female healthy individuals,and the concentration of IL-17 in plasma and culture supernatant was measured by ELISA.The difference of expression level of miR-146a、miR-23b and IL-17 in RA patients and healthy individuals was analyzed.Collected the clinical laboratory indexes of RA patients including ESR,CRP,anti CCP antibody,RF and calculating the score of DAS28,the correlation between miR-146a and miR-23b with IL-17,ESR,CRP,anti CCP antibodies,RF and DAS28 were analyzed,respectively.In addition,the levels of miR-146a,miR-23b and IL-17 were also detected after PBMCs treatment by estradiol between RA group and control group,and the differences were analyzed.Results:The expression levels of miR-146a and miR-23b in female RA patients increased compared to those in the healthy individuals(P<0.05).There was no significant correlation between miR-146a or miR-23b with IL-17,ESR,CRP,anti CCP antibody,RF and DAS28(P>0.05).After estrogen treatment,there was no significant change in miR-146a and miR-23b in female RA patients,the concentration of IL-17 in supernatant in female RA patients was significantly increased(P<0.05),and the expression level of miR-146a in healthy individuals was significantly down-regulated(P<0.05),the expression level of miR-23b did not change significantly(P>0.05).Conclusion:The expression levels of miR-146a and miR-23b in female RA patients are up-regulated.Estrogen may to be involved in the developement of RA by up-regulation IL-17.On the other hand,estrogen may prevent healthy people from developing to RA by down-regulation of miR-146a in the PBMCs in healthy individuals.
作者
祝静
晏波
蒋瑶
王东生
邢艳
ZHU Jing;YAN Bo;JIANG Yao;WANG Dong-sheng;XING Yan(Department of Laboratory Medicine,North Sichuan Medical College;Department of Clinical Laboratory,Affiliated Hospital of North Sichuan Medical College;Transforming Medicine Research Centre,North Sichuan Medical College,Nanchong 637000;Department of Laboratory,Sichuan Cancer Hospital,Chengdu 610000,Sichuan,China)
出处
《川北医学院学报》
CAS
2020年第4期637-641,共5页
Journal of North Sichuan Medical College
基金
四川省学术和技术带头人培养基金(740/74130801)。
