摘要
目的研究大黄素促进人结肠癌细胞HCT116细胞凋亡的作用及其分子机制。方法通过CCK8法检测大黄素对细胞活力的影响,并计算出IC50。Annexin-V/PI流式细胞术检测细胞凋亡,流式细胞术检测细胞周期和荧光探针DCF-DA细胞内活性氧(ROS)的水平,Western blot法检测Bax、Bcl-2、p-ERK1/2、ERK1/2和c-Myc的表达。结果不同浓度的大黄素抑制HCT116细胞的活力,并且具有浓度依赖性。大黄素将HCT116细胞周期阻滞在G0/G1期(P<0.05),并且能够明显诱导ROS的产生(P<0.01),Western blot结果显示大黄素能够引起Bax/Bcl-2表达的上调,p-ERK1/2和c-Myc表达的降低(P<0.05)。结论大黄素可以通过上调Bax/Bcl-2,下调c-Myc和p-ERK/ERK的表达从而促进结肠癌细胞的凋亡,阻滞细胞周期和增加ROS的产生。
OBJECTIVE To investigate the effect of emodin on human colon cancer cell line HCT116 and its molecular mechanism.METHODS The inhibitory effects of emodin on the viability of HCT116 cells were measured by CCK8 assay.Apoptosis rates of HCT116 cells were determined by flow cytometry.The cell cycle and the level of reactive oxygen species(ROS)in the fluorescent probe DCF-DA were detected by flow cytometry.The protein levels of Bax,Bcl-2,p-ERK1/2,ERK1/2 and c-Myc were determined by Western blot.RESULTS Treatment with emodin of 0,25,50,100,200,300μmol/L for 24 h significantly reduced the viability of HCT116 cells.Emodin promoted the apoptosis of HCT116 cells,blocked cell growth cycle in G0/G1 phase and induced the production of ROS,significantly.Western blot results showed that emodin promoted the up-regulation of Bax/Bcl-2 expression and inhibited the expression of c-Myc and p-ERK/ERK.CONCLUSION Emodin inhibits the proliferation of HCT116 cells,promotes cell apoptosis,induces cell cycle arrest in G1/G0 phase and ROS production,by promoting Bax/Bcl-2 expression and inhibiting c-Myc,p-ERK/ERK expression.
作者
张昊悦
赵蓓
章阳
ZHANG Hao-yue;ZHAO Bei;ZHANG Yang(Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210001, China;Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200437, China)
出处
《南京中医药大学学报》
CAS
CSCD
北大核心
2020年第4期485-488,共4页
Journal of Nanjing University of Traditional Chinese Medicine
基金
“十三五”南京市卫生青年人才培养工程(NWQR-201703)。
