摘要
【目的】研究小麦ZY96-3籽粒在灌浆过程中与籽粒发育相关基因的表达模式,为了解基因调控籽粒灌浆的分子机制提供参考。【方法】采用实时荧光定量PCR(qRT-PCR)技术,分析小麦ZY96-3籽粒灌浆期间6个与籽粒发育相关基因的表达动态。【结果】从小麦ZY96-3开花后6个目标基因的表达模式均是先增后降,且都在花后7 d开始表达;与粒重相关基因TaTGW6在花后7 d表达量最高,与籽粒大小相关基因TaCYP78A5、蛋白质合成基因TaPBF-D和淀粉合成酶基因GBSSⅠ、SBE1、AGPase1都在花后15 d左右表达量达到最大;自花后19 d开始,各基因的表达量均显著下降。【结论】与粒重相关基因TaTGW6主要在小麦ZY96-3籽粒灌浆初期起关键作用,而其余5个基因主要在籽粒灌浆中后期发挥功能。基因在小麦ZY96-3籽粒灌浆期间的表达模式。
【Objective】To explore the expression pattern of ZY96-3 genes related to grain development during grain filling stage.in order to provide reference for further understanding of the molecular mechanism of gene regulation in grain filling.【Method】The real-time fluorescence quantitative PCR(qRT-PCR)technique was used to analyze the expression dynamics of 6 genes related to grain development during grain filling stage of ZY96-3.【Result】The results showed that the expression patterns of six target genes of ZY96-3 increased first and then decreased,and all of them began to express 7 days after anthesis;TaTGW6 genes were involved in grain weight on the 7 d after flowering to express the highest amount,and the grain size TaCYP78A5 related genes,protein synthesis genes TaPBF-D and starch synthase gene GBSSⅠ,SBE1,AGPase1 all around 15 d after flowers express maximum amount;However,the expression level of each gene decreased significantly at 19 d after anthesis.【Conclusion】This study showed that the grain-weight related gene TaTGW6 mainly played a key role in the early stage of wheat ZY96-3 grain filling,while the other 5 genes mainly played a role in the middle and late stage of wheat grain filling.
作者
杨帅
任明见
李振华
李鲁华
徐如宏
YANG Shuai;REN Mingjian;LI Zhenhua;LI Luhua;XU Ruhong(Guizhou Sub-center of National Wheat Improvement Center/College of Agronomy,Guizhou University,Guiyang 550025,China)
出处
《新疆农业科学》
CAS
CSCD
北大核心
2020年第7期1187-1194,共8页
Xinjiang Agricultural Sciences
基金
国家自然科学基金(31660390)
贵州省农业成果转化计划(黔科合成果(2016)4022号)
国家“七大农作物育种”重点专项(2017YFD0100900)。
关键词
小麦
灌浆期
基因表达
实时荧光定量PCR
wheat
filling stage
gene expression
real-time fluorescence quantitative PCR
