摘要
目的探讨胃癌组织中富含亮氨酸重复序列G-蛋白偶联受体5(LGR5)、缺氧诱导因子1α(HIF-1α)的阳性表达情况与相关性,以及与胃癌患者临床病理特征的关系。方法选取2019年1月—2019年12月徐州医科大学附属医院肿瘤外科收治的胃癌患者的临床资料,共包含126例胃癌组织及105例随机选择的癌旁组织。采用免疫组化法检测胃癌组织及癌旁组织中LGR5、HIF-1a的表达,比较两组的LGR5、HIF-1a阳性表达率,分析二者的相关性及其与胃癌患者临床病理特征的关系。结果LGR5、HIF-1α在胃癌组织中的阳性率均显著高于癌旁组织(P<0.05)。LGR5、HIF-1α的阳性表达率与胃癌患者的性别、年龄和肿瘤部位无明显相关性(P>0.05),而与肿瘤大小、分化程度、TNM分期和淋巴结转移明显相关(P<0.05)。进一步相关性分析结果显示,胃癌组织中LGR5与HIF-1α的表达呈正相关(r=0.389,P<0.05)。结论LGR5、HIF-1α在胃癌组织中的阳性率均显著高于癌旁组织;胃癌组织中LGR5、HIF-1α的表达与患者的临床病理特征相关;胃癌组织中LGR5与HIF-1α的表达呈正相关。
Objective To investigate the expression of leucine-rich repeat-containing G-protein coupled receptor 5(LGR5)and hypoxia inducible factor 1α(HIF-1α)in gastric cancer tissues and their correlation with the clinical pathological features.Methods Clinical data were selected of gastric cancer patients who were admitted to the tumor surgery department of the Affiliated Hospital of Xuzhou Medical University between January 2019 and December 2019,including 126 gastric cancer tissues and 105 randomly selected para-carcinoma tissues.Immunohistochemistry was used to detect the expressions of LGR5 and HIF-1a.The positive expressions of LGR5 and HIF-1a in the two groups were compared,and their correlation with each other was analyzed.The relationship of LGR5 and HIF-1αexpressions with clinicopathological features of gastric cancer patients was also analyzed.Results The positive rates of LGR5 and HIF-1αwere found higher in gastric cancer tissues than in para-carcinoma tissues(P<0.05).There was no significant relations between the positive expression rates of LGR5 and HIF-1αand the gender,age and tumor location of gastric cancer patients(P>0.05).But the positive expression rates of LGR5 and HIF-1αwere significantly correlated with tumor sizes,differentiation degrees,TNM stages and lymph node metastasis of the patients(P<0.05).Further correlation analysis showed that there was a positive correlation between LGR5 and HIF-1αexpressions in gastric cancer tissues(r=0.389,P<0.05).Conclusion The positive rates of LGR5 and HIF-1αwere higher in gastric cancer tissues than in para-carcinoma tissues.The expressions of LGR5 and HIF-1αin gastric cancer tissues were related to the clinicopathological characteristics of gastric cancer.The expression of LGR5 was positively correlated with the expression of HIF-1αin gastric cancer tissues.
作者
高国安
高超
GAO Guo'an;GAO Chao(Department of Oncology,Graduate School,Xuzhou Medical University,Xuzhou,Jiangsu,221000,China;Department of Oncology,the Affiliated Hospital of Xuzhou Medical University,Xuzhou,Jiangsu,221000,China)
出处
《肿瘤药学》
CAS
2020年第4期456-460,466,共6页
Anti-Tumor Pharmacy
