摘要
目的对2018年从产地和市售贝类样品中分离的140株副溶血性弧菌的毒力基因和耐药性进行分析。方法采用聚合酶链式反应(PCR)扩增法检测毒力基因和耐药基因,采用药敏纸片法测试菌株的耐药性。结果140株副溶血性弧菌均不含有tdh基因,1株菌含有trh基因;所有菌株对氨苄西林耐药,部分菌株对头孢唑啉、头孢呋辛钠、链霉素、阿米卡星、复方新诺明、四环素和氟苯尼考耐药,所有菌株对头孢吡肟、庆大霉素、亚胺培南、美罗培南、氧氟沙星和强力霉素敏感,6株菌呈现耐受2类及以上抗菌药的多重耐药性;耐链霉素菌株检出耐药基因strA、strB,耐四环素菌株检出耐药基因tetA,耐复方新诺明菌株检出耐药基因sul2,耐氟苯尼考菌株检出耐药基因floR。结论大多数副溶血性弧菌不含有毒力基因,菌株呈现不同程度的耐药性,少量菌株含有多个耐药基因,表现出多重耐药性,提示应继续加强水产品中副溶血性弧菌的致病性和耐药性监测。
Objective To analyze the virulence genes and antimicrobial resistance of 140 Vibrio parahaemolyticus strains from shellfish collected from farms and markets.Methods Virulence genes and antimicrobial resistance genes were detected by PCR amplification method,and antimicrobial resistance was determined by disk diffusion test.Results None of the 140 strains carried tdh gene,and trh gene was detected in one strain.All strains were resistant to ampicillin,some strains were resistant to cefazolin,cefuroxime sodium,streptomycin,amikacin,sulphamethoxazole/trimethoprim,tetracycline and florfenicol.All strains were sensitive to cefepime,gentamicin,imipenem,meropenem,ofloxacin and doxycycline.Six strains showed multi-antimicrobial resistance to two or more categories of antimicrobials.The resistance genes strA and strB were detected in streptomycin resistant strains,tetA was detected in tetracycline resistant strains,sul2 was detected in sulphamethoxazole/trimethoprim resistant strains,and floR was detected in florfenicol resistant strain.Conclusion Most of Vibrio parahaemolyticus strains didn’t carry virulence genes and showed antimicrobial resistance to a certain extent.Some strains had more than one antimicrobial resistance gene,which exhibited multi-antimicrobial resistance.These results indicated that enhancing the monitoring of pathogenicity and antimicrobial resistance of Vibrio parahaemolyticus in aquatic products should be continued.
作者
李丽倩
姚琳
林才云
李风铃
曲梦
王联珠
翟毓秀
牟海津
江艳华
LI Liqian;YAO Lin;LIN Caiyun;LI Fengling;QU Meng;WANG Lianzhu;ZHAI Yuxiu;MU Haijin;JIANG Yanhua(Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality,Ministry of Agriculture and Rural Affairs,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shandong Qingdao 266071,China;College of Food Science and Technology,Ocean University of China,Shandong Qingdao 266003,China)
出处
《中国食品卫生杂志》
CSCD
北大核心
2020年第4期364-370,共7页
Chinese Journal of Food Hygiene
基金
国家自然科学基金项目(31601566)
国家重点研发计划课题(2017YFC1600703)
现代农业产业技术体系专项(CARS-47)。
关键词
副溶血性弧菌
毒力基因
耐药性
耐药基因
Vibrio parahaemolyticus
virulence gene
antimicrobial resistance
antimicrobial resistance gene
