摘要
【目的】筛选抗旱高光效的油茶种质,为后续油茶耐旱品种选育和引种栽培提供参考。【方法】以我国华南地区广泛种植的岑软2号、岑软3号、桂无1号和桂无4号共4个油茶优良品种为试验材料,设置PEG 6000(10%、20%、30%)3个干旱胁迫强度,给予12、24、36 h的胁迫处理和12 h的复水处理,测定叶片叶绿素含量和叶绿素荧光参数,并采用隶属函数法和聚类分析法综合评价4个油茶品种的抗旱性。【结果】干旱胁迫下,4个油茶品种叶片的叶绿素含量和叶绿素荧光参数均发生明显变化。其中,岑软2号和岑软3号叶片的叶绿素a和叶绿素b含量稳定;桂无1号叶片叶绿素a含量及桂无4号叶片叶绿素a和叶绿素b含量均显著降低。4个油茶品种的非光化学淬灭(NPQ)和PSⅡ调节性能量耗散(Y NPQ)均上升,其中岑软2号和岑软3号叶片的NPQ和Y NPQ值较大且增幅明显,表明其抗旱能力较强。岑软2号和岑软3号叶片的表观电子传递速率(ETR)和实际光量子效率(Y PSⅡ)较大,且随胁迫持续逐渐升高,PSⅡ非调节性能量耗散(Y NO)波动持平,甚至下降;桂无1号和桂无4号叶片的ETR、Y PSⅡ和Y NO变化与岑软2号和岑软3号相反,表明其抗旱能力较弱。复水处理后,岑软2号和岑软3号叶片叶绿素荧光参数有恢复迹象,尤其是在轻度胁迫下ETR、Y PSⅡ、Y NO、NPQ和Y NPQ恢复至对照水平,表明其具有良好的旱后恢复能力;桂无1号和桂无4号相关指标未有改善,表明其抗旱能力较弱。【结论】结合隶属函数法和聚类分析法,4个油茶品种可划分为2类,其中岑软2号和岑软3号为抗旱性较强的品种,桂无1号和桂无4号为抗旱性较弱的品种,按照抗旱性由强到弱排序依次为岑软2号、岑软3号、桂无1号、桂无4号。
【Objective】The selection of Camellia oleifera with favorable photosynthetic efficiency was studied aiming to provide some references for breeding of C.oleifera cultivars with drought tolerance,and promote the healthy development of C.oleifera industry.【Method】Photosynthetic pigments and chlorophyll fluorescence indices of four C.oleifera cultivars including‘Cenruan2’(C2),‘Cenruan 3’(C3),‘Guiwu 1’(G1)and‘Guiwu 4’(G4)were observed under different PEG 6000 concentrations(10%,20%,30%)and different stress time(12 hours,24 hours,36 hours)followed by 12 hours rewatering.The subordinate function analysis and cluster analysis were used to evaluate the drought tolerances of four cultivars comprehensively.【Result】The photosynthetic pigments and chlorophyll fluorescence indices were changed accordingly stressed by drought.The contents of chlorophyll a and chlorophyll b were stable in C2 and C3;The content of chlorophyll a in G1,and the contents of chlorophyll a and chlorophyll b in G4 decreased significantly.Non-photochemical quenching(NPQ)and the quantum yield of regulated energy dissipation(Y NPQ)of four cultivars increased,among which,C2 and C3 increased significantly indicating their stronger drought resistance.The electron transport rate(ETR)and active quantum efficiency of PSII(Y PSⅡ)of C2 and C3 increased gradually,and the quantum yield of non-regulated energy dissipation(Y NO)decreased slightly.The changes of ETR,Y PSⅡand(Y NO)of G1 and G4 were converse process to C2 and C3,which means G1 and G4 with less drought stress resistance.Most of chlorophyll fluorescence indices of C2 and C3 generally recovered after rewatering,especially under light drought stress.G1 and G4 did not recovered reflecting less drought resistant ability.【Conclusion】The results of subordinate function analysis and cluster analysis showed that four C.oleifera cultivars were classified into two classes,in which,‘Cenruan 2’and‘Cenruan 3’could be classified as drought resistant cultivars,meanwhile,‘Guiwu 1’and‘Guiwu 4’as drought sensitive cultivars.Four cultivars were ordered as‘Cenruan 2’>‘Cenruan 3’>‘Guiwu 1’>‘Guiwu 4’according to drought resistance ability.
作者
董斌
蓝来娇
黄永芳
韦雪芬
龚海光
洪文泓
黄丽英
陈厚蓉
DONG Bin;LAN Laijiao;HUANG Yongfang;WEI Xuefen;GONG Haiguang;HONG Wenhong;HUANG Liying;CHEN Hourong(Guangdong Agriculture Industry Business Polytechnic,Guangzhou 510507,Guangdong,China;College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou 510642,Guangdong,China;Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm,South China Agricultural University,Guangzhou 510642,Guangdong,China;South China Botanical Garden,Chinese Academy of Sciences,Guangzhou 510650,Guangdong,China;Foshan University,Foshan 5238231,Guangdong,China)
出处
《经济林研究》
北大核心
2020年第3期16-25,共10页
Non-wood Forest Research
基金
广东省大学生科技创新培育专项资金资助项目(Pdjh2019b0797)
广东省林业科技创新项目(2017KJCX005)
广东农工商职业技术学院热带作物应用技术协同创新中心建设项目(XJZX1902)。
关键词
油茶
干旱胁迫
叶绿素
叶绿素荧光
综合评价
Camellia oleifera
drought stress
chlorophyll
chlorophyll fluorescence
comprehensive evaluation
