摘要
通过网络药理学的研究方法,探索"黄芪―白术"治疗肺癌的可能作用机制。使用中药系统药理学分析平台(TCMSP)数据库查询"黄芪―白术"的活性成分,运用TCMSP数据库对活性成分的靶点进行筛选,并利用Uniprot数据库对靶点进行标准化命名。通过"TTD"数据库、"DisGeNET"数据库和"OMIM"数据库查询肺癌相关靶点。利用Venn Diagram得到"化合物―疾病"共同靶点。运用STRING数据库进行蛋白质互作网络(PPI)的构建。使用R语言进行GO富集分析和KEGG通路富集分析。"黄芪―白术"药对共筛选得到活性成分19个,潜在作用靶点180个。治疗肺癌的预测靶点29个,主要涉及AKT1、VEGFA、EGFR等。GO功能富集分析,按照P<0.05进行筛选,涉及生物过程1260个,细胞组分12个,分子功能32个,主要涉及活性氧代谢过程、类固醇激素反应、凋亡信号通路的调控、T细胞活化等。KEGG信号通路富集分析,按照P<0.05进行筛选,涉及信号通路26个,主要涉及PI3K-Akt信号通路、EGFR酪氨酸激酶抑制剂耐药性、铂类耐药性、HIF-1信号通路、MAPK信号通路、Rap1信号通路、Ras信号通路等。"黄芪―白术"通过调控信号通路、抗癌药物耐药性等发挥抑制肿瘤血管生成、诱导肿瘤细胞凋亡、抑制肿瘤转移等作用,从而治疗肺癌。"黄芪―白术"在治疗肺癌的过程中涉及到多活性成分、多靶点及多信号通路。
The study aimed to explore the possible mechanism of"Astragalus membranaceus-Atractylodes macrocephala koidz"for the treatment of lung cancer through network pharmacology.The active ingredients of"Astragalus membranaceus-Atractylodes macrocephala koidz"were queried through the TCMSP database,the targets of the active ingredients were screened through the TCMSP database,and the targets were standardized and named through the Uniprot database.The lung cancer related targets were queried through the"TTD"database,"DisGeNET"database and"OMIM"database.The"compounddisease"common targets were obtained using Venn Diagram,and the protein interaction network(PPI)was constructed using STRING database,and R language was used for GO enrichment analysis and KEGG pathway enrichment analysis."Astragalus membranaceus-Atractylodes macrocephala koidz"drugs were screened to obtain 19 active ingredients,180 potential targets,29 potential targets for the treatment of lung cancer,including AKT1,VEGFA,EDFR,etc.GO functional enrichment was analyzed and screened according to P<0.05,which involved 1260 biological processes,12 cell groups,32 molecular functions,such as reactive oxygen species metabolic process,response to steroid hormone,regulation of apoptotic signaling pathway,T cell activation,etc.KEGG signal pathway enrichment was analyzed and screened according to P<0.05,involving 26 signal pathways,including PI3 K-Akt signaling pathway,EGFR tyrosine kinase inhibitor resistance,Platinum drug resistance,HIF-1 signaling pathway,MAPK signaling pathway,Cellular senescence,Rap1 signaling pathway,Ras signaling pathway,etc."Astragalus membranaceus-Atractylodes macrocephala koidz"plays a role in inhibiting tumor angiogenesis,inducing tumor cell apoptosis and inhibiting tumor metastasis by regulating signaling pathways and anticancer drug resistance,thereby treating lung cancer."Astragalus membranaceus-Atractylodes macrocephala koidz"involves multiple active ingredients,multiple targets and multiple signaling pathways in the treatment of lung cancer.
作者
刘梓燊
杨国旺
LIU Zishen;YANG Guowang(Department of Oncology,Beijing Hospital of Traditional Chinese Medicine,Capital Medical University,Beijing 100010,China;Capital Medical University,Beijing 100069,China)
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2020年第5期134-143,共10页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家“十三五”重大新药创制专项课题(2019ZX09734001)
国家重点研发计划项目(2018YFC1705101)
2019年重大疑难疾病中西医临床协作能力建设项目(肝癌)。
关键词
网络药理学
肺癌
黄芪―白术
作用机制
network pharmacology
lung cancer
Astragalus membranaceus-Atractylodes macrocephala koidz
mechanism of action
