摘要
背景:牙本质颗粒、磷酸三钙/胶原蛋白复合材料和Bio-oss颗粒均可以修复颌骨缺损,但是其成骨效果的优异并不明确。目的:比较3种不同骨移植材料在比格犬下颌骨缺损中的成骨效果。方法:选取8只1岁龄比格犬,在双侧下颌外斜线处制备10 mm×8 mm×2 mm的箱状骨缺损区,随机分为4组,每组4个骨缺损模型:A组植入未脱矿牙本质颗粒,B组植入磷酸三钙/胶原蛋白复合材料,C组植入Bio-oss颗粒,D组作为空白对照。移植3个月后取材,进行组织学观察。实验方案获得大连医科大学实验动物中心伦理委员会批准。结果与结论:①A组牙本质颗粒略有吸收,材料周围有新生骨组织包绕,可见新生骨小梁和毛细血管,植骨区中心区域可见牙本质颗粒被大量纤维结缔组织包绕;B组可见少量的新生骨小梁和成骨细胞,在纤维结缔组织中可见大量粉末状β-磷酸三钙颗粒和少量的炎症细胞浸润,部分β-磷酸三钙颗粒被新生骨组织包绕,新生骨中可见骨髓腔;C组可见部分Bio-oss颗粒被新生骨组织包绕,部分Bio-oss颗粒被周围的纤维结缔组织包裹,纤维、颗粒和新生骨交织在一起;D组并未见新骨形成,大量的纤维结缔组织中可见许多毛细血管;②A、B、C组的新骨生成率均高于D组(P <0.05),A、C组高于B组(P <0.05);③结果表明3种骨移植材料都能促进新骨的形成,未脱钙异种牙本质颗粒与Bio-oss颗粒的短期成骨效果无差异,均优于磷酸三钙/胶原蛋白复合材料,但远期效果还需进一步观察。
BACKGROUND: Dentin particles, tricalcium phosphate/collagen protein composites and Bio-oss particles can repair jaw defects, but the excellent osteogenic effect is not clear.OBJECTIVE: To compare osteogenic effects of three different bone graft materials on mandibular defects in beagle dogs.METHODS: Eight 1-year-old beagles were selected. A boxed bone defect area of 10 mm × 8 mm × 2 mm was prepared at the bilateral mandibular external oblique line and randomly divided into four groups with four bone defect models in each group. Undemineralized dentin particles were implanted in group A;tricalcium phosphate/collagen protein composite materials were implanted in group B;Bio-oss particles were implanted in group C;and group D was used as blank control. Three months after transplantation, the samples were taken for histological observation. The experimental animals were approved by the Ethics Committee of the Experimental Animal Center of Dalian Medical University.RESULTS AND CONCLUSION:(1) In group A, dentin particles were slightly absorbed, surrounded by new bone tissue;new bone trabeculae and capillaries could be seen, and a large number of fibrous connective tissue surrounded dentin particles in the central area of bone graft. In group B, a small number of new bone trabeculae and osteoblasts could be seen;a large number of powdered β-tricalcium phosphate particles and a small amount of inflammatory cells could be seen in the fibrous connective tissue;and some β-tricalcium phosphate particles were surrounded by new bone tissue. Bone marrow cavity could be seen in the new bone. In group C, some Bio-oss particles were surrounded by new bone tissue;some Bio-oss particles were wrapped by surrounding fibrous connective tissue, and fibers, particles and new bone were intertwined. There was no new bone formation in group D, and many capillaries could be seen in a large number of fibrous connective tissue.(2) The rate of new bone formation in groups A, B and C was higher than that in group D(P < 0.05);the rate in groups A and C was higher than that in group B(P < 0.05).(3) The results show that all the three kinds of bone graft materials can promote the formation of new bone. The short-term osteogenic effects of undecalcified xenogeneic dentin particles and Bio-oss particles are better than tricalcium phosphate/collagen protein composites, but the long-term effects need to be further observed.
作者
赵彬彬
仲维剑
马国武
李永奇
王宁
Zhao Binbin;Zhong Weijian;Ma Guowu;Li Yongqi;Wang Ning(School of Stomatology,Dalian Medical University,Dalian 116044,Liaoning Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2021年第10期1507-1511,共5页
Chinese Journal of Tissue Engineering Research
基金
辽宁省自然科学基金(2015020313),项目负责人:仲维剑。
关键词
骨
材料
骨移植
骨再生
牙本质颗粒
磷酸三钙
胶原蛋白
复合材料
Bio-oss颗粒
动物实验
bone
materials
bone transplantation
bone regeneration
dentin granules
tricalcium phosphate
collagen protein
composite materials
Bio-oss particles
animal experiment
