摘要
以优质两系不育系Y58S与携有多个抗病基因的优质保持系M215B杂交,F1抽穗期再与优质两系抗病不育系RGD-7S复交,通过分子标记辅助选择技术和系谱法育种相结合,选育出同时携有Pi-2、Pi-1、Pi-Kh、Xa21和Xa23的两系不育系禾9S。稻瘟病抗性表明,禾9S田间鉴定平均叶瘟发病率仅为2.5%(感病对照为83.0%),病情指数为0.6(感病对照为52.9),表现抗苗瘟;穗颈瘟平均发病率为38.3%(感病对照为88.5%),病情指数为8.0(感病对照为62.3),表现中抗穗颈瘟;室内鉴定抗菌株率为87.5%(感病对照为6.25%),表现为抗稻瘟病;田间和室内鉴定综合评价为中抗稻瘟病。白叶枯接菌鉴定表明,禾9S在用PX099、ZHE173和GD1358 3个菌株接菌14 d后的病斑长度分别为0.1 cm、0.1 cm和0.4 cm,与抗性供体亲本M215B相近(病斑长度分别为0.1 cm、0.1 cm和0.3 cm),明显短于对照品种RGD-7S(病斑长度分别为2.2 cm、7.4 cm和7.8 cm)和Y58S(病斑长度分别为1.3 cm、5.1 cm和5.5 cm)的病斑长度。主要特征特性观察表明,禾9S具育性转换起点温度低(23℃左右)、可繁性好、柱头外露率高、米质优等特点,目前利用禾9S已配组出禾两优676等10多个杂交稻新品种,其中禾两优676和禾两优639表现生育期适中、产量高、抗性好、稳产性好等特点,已分别于2019年5月和2020年6月通过福建省农作物品种审定委员会审定,另有多个品种已进入各级续试或生产试验,表明该不育系具较好的推广应用前景。
A good-quality two-line male sterile line(Y58 S)was crossed with a good-quality maintainer line(M215 B)that carry multiple disease resistant genes. The obtained F1 hybrids were crossed with RGD-7 S,which is a good-quality and disease-resistant male sterile line at the heading stage,a two-line male sterile line(He 9 S)that contained five resistance genes,Pi-2,Pi-1,Pi-Kh,Xa21 and Xa23 was bred by marker-assisted selection in combination with pedigree breeding. The average incidence rate of leaf blast in the field identification of He 9 S was only 2.5%(83.0% for the susceptible control),and the disease index of leaf blast was only 0.60(52.9 for the susceptible control). The incidence rate of panicle neck blast of He 9 S was 38.3%(88.5% for the susceptible control)and the disease index of panicle neck blast was 8.0(62.3 for the susceptible control). The Frequency of resistance of He 9 S in indoor artificial inoculation was 87.5%(6.25 for the susceptible control). Tests for rice blast resistance under greenhouse condition and field conditions showed moderate resistant to rice blast in He 9 S. Furthermore,inoculation with three respective isolates(PXO99,ZHE173 and GD1358)showed that the diseased lesion length of He 9 S was variable between 0.1-0.4 cm,similarly to the resistant donor parent,M215 B,the lesion length of which were 0.1 cm,0.1 cm and 0.3 cm respectively. On the contrast,the lesion length of both control varieties RGD-7 S and Y58 S were variable from 1.3 cm to 7.8 cm. Attractively,He 9 S showed low fertility conversion temperature(about 23 ℃),good versatility,high stigma exsertion rate and excellent rice quality. Up to date,>10 hybridization combinations have been derived from He 9 S. For example,two elite variety Heliangyou 676 and Heliangyou 639,which exhibited moderate growth period,high yield,good resistance and good stability,had been approved and released to farmers in May 2019 and in June 2020 respectively in Fujian province,and there are more than 3 varieties are in second-year tests or production test. Taken together,this study released an elite sterile line with a predictable potential in breeding for elite rice hybrids.
作者
陈志伟
官华忠
毛大梅
潘润森
周元昌
吴为人
CHEN Zhi-wei;GUAN Hua-zhong;MAO Da-mei;PAN Run-sen;ZHOU Yuan-chang;WU Wei-ren(Fujian Agriculture and Forestry University/Fujian Provincial Key Laboratory,of Crop Breeding by Design,Fuzhou 350002)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2020年第5期1078-1088,共11页
Journal of Plant Genetic Resources
基金
国家重点研发项目(2017YFD0100100)
福建省区域发展项目(2018N3011)
福建省科技引导项目(2018N006)~~
关键词
水稻
抗稻瘟病
抗白叶枯病
基因聚合
分子标记辅助选择
rice
resistance to rice blast
resistance to bacterial blight
gene polymerization
marker-assisted selection
