摘要
目的评价白藜芦醇预处理对短期多次七氟烷吸入诱发老龄大鼠神经认知障碍的影响。方法选取健康老龄雄性SD大鼠60只,体重310~460 g,月龄18个月,将实验动物随机分为对照组(C组,n=20)、七氟烷组(S组,n=20)和白藜芦醇预处理组(R组,n=20)。R组予白藜芦醇40 mg·kg^-1经口灌胃,1次·d^-1,持续7 d,C组和S组予等容量1%羟甲基纤维素钠溶液经口灌胃;S组和R组于灌胃后吸入3%七氟烷,C组大鼠吸入空气,100 min·d^-1,持续5 d。末次七氟烷吸入结束后立即处死大鼠,取其海马组织,测定海马内超氧化物岐化酶(SOD)活性、微量丙二醛(MDA)含量和IL-1β含量。末次七氟烷吸入结束后24 h行Morris水迷宫实验测试大鼠认知功能,记录大鼠逃避潜伏期时间和原平台位置停留时间。结果与C组比较,S组逃避潜伏期时间明显延长,原平台位置停留时间明显缩短;海马IL-1β、MDA含量明显升高,SOD活性明显下降(P<0.05);与S组比较,R组逃避潜伏期明显缩短,原平台位置停留时间明显延长,海马IL-1β、MDA含量明显降低,SOD活性明显升高(P<0.05)。结论白藜芦醇预处理可以改善老龄大鼠短期多次七氟烷麻醉诱发的神经认知障碍,其机制可能与白藜芦醇改善大鼠颅脑组织抗氧化效能及抑制海马炎性瀑布反应有关。
Objective To investigate the effects of resveratrol pretreatment on neurocognitive disorder induced by repeated sevoflurane anesthesia in aged rats.Methods Sixty healthy male Sprague-Dawley rats aged 18 months(310-460 g)were randomly divided into three groups,with 20 rats in each group.Group R was gavaged with resveratrol 40 mg·kg^-1 once daily for 7 days before the first inhalation of sevoflurane.Groups C and S were orally given the equal volume of 1%sodium hydroxymethyl cellulose solution.After gavage,groups S and R inhaled 3%sevoflurane inhalation but group C inhaled air(100 min·d^-1)for 5 consecutive days.Rats were sacrificed immediately after sevoflurane inhalation and hippocampi were harvested to determine interleukin-1β(IL-1β)content,superoxide dismutase(SOD)activity and malondialdehyde(MDA)level.Rats’cognitive function was assessed using Morris water maze at 24 h after the inhalation of sevoflurane,to record the escape latency and the time spent in the original platform.ResultsCompared with group C,the escape latency was prolonged,the time spent in original platform was shorted,the contents of IL-1βand MDA were increased,and the activity of SOD was decreased in group S(P<0.05).Compared with group S,the escape latency was shortened,the time spent in original platform was prolonged,the contents of IL-1βand MDA were decreased,and the activity of SOD was increased in group R(P<0.05).Conclusion Resveratrol pretreatment can improve neurocognitive disorder induced by repeated sevoflurane anesthesia in aged rats,and the mechanism may be involved in improvement of antioxidant capacity in brain tissue and inhibition of inflammatory responses in hippocampus.
作者
谭兰兰
朱小芳
李坤
胡小冬
陈世彪
TAN Lan-lan;ZHU Xiao-fang;LI Kun;HU Xiao-dong;CHEN Shi-biao(Medical Department of Graduated School,Nanchang University,Nanchang 330006,China;Department of Anesthesiology,the First Affiliated Hospital,Nanchang University,Nanchang 330006,China;Department of Anesthesiology,Xinyu People’s Hospital;Department of Anesthesiology,Xinyu Mining Hospital,Xinyu 338000,China)
出处
《南昌大学学报（医学版）》
CAS
2020年第4期28-31,共4页
Journal of Nanchang University：Medical Sciences
关键词
白藜芦醇
七氟烷
抗氧化
神经认知障碍
动物
实验
大鼠
老龄
resveratrol
sevoflurane
antioxidant
neurocognitive disorder
animal,laboratory
rats,aging
