摘要
目的探讨实验动物小鼠不同组织冰冻切片制作的方法与技巧,提高冰冻切片质量,更好地服务于单抗药物非临床安全性评价。方法采用德国徕卡CM1950恒温冷冻切片机,对健康NIH小鼠心、肝、脾、肺、肾、脑组织进行取材、包埋、冰冻切片、HE染色、封片。结果切片无褶皱、无刀痕、无破损,镜下组织无过多冰晶,细胞形态清晰,细胞核和细胞质颜色分明,染色良好。结论在制作冰冻切片时,应该从取材、包埋、速冻、温度选择、恒温冷冻切片等各个环节进行严格控制,但是,不同组织根据自身特点会存在差异,应区别对待。此外,在掌握关键性技术要点的同时还应该具有高度的责任心和耐心,制作出高质量的冰冻切片,才能更好地服务于单抗药物非临床安全性评价工作。
Objective We explored the method and techniques of preparing frozen sections using different mice tissues as our experimental animal materials,to improve frozen section quality,and to increase the serviceability of these techniques for the non-clinical safety evaluation of monoclonal antibodies.Methods The heart,liver,spleen,lung,kidney,and brain tissues of healthy NIH mice were collected,embedded,frozen,and sliced using a Leica CM1950 thermostatic frozen microtome,and then stained by hematoxylin and eosin dye and sealed.Results The slices were observed by light microscopy,and the result show no fold,no knife marks,no damage,and no excessive ice crystals.The cell morphology was clear,with the nucleus and cytoplasm clearly colored,and staining was good.Conclusions In the process of making frozen slices,strict controls from the aspects of collecting,embedding,quick-freezing,temperature selection,and thermostatic frozen sectioning should be carried out.However,different tissues have differences according to their own characteristics,and should be treated differently.In addition,to prepare high-quality frozen sections,and to better serve the non-clinical safety evaluation of monoclonal antibodies,we collectively need a high sense of responsibility and patience while mastering key technical points.
作者
王园园
王涛
李新
王斌
江魁
WANG Yuanyuan;WANG Tao;LI Xin;WANG Bin;JIANG Kui(Hualan Genetic Engineering CO.,LTD.,XinXiang 453500,China)
出处
《中国比较医学杂志》
CAS
北大核心
2020年第9期59-63,共5页
Chinese Journal of Comparative Medicine
关键词
小鼠
非临床研究
冰冻切片
实验动物
新鲜组织
mice
non-clinical studies
frozen section
experimental animals
fresh tissue
