摘要
目的建立基于表面增强拉曼光谱(SERS)技术的金黄色葡萄球菌检测方法。方法在碳二亚胺催化下,氨基化玻片与万古霉素形成共价结合,用紫外分光光度计测量玻片偶联万古霉素含量。利用万古霉素修饰玻片捕获并固定金黄色葡萄球菌,在银纳米颗粒增强下,用激光显微拉曼光谱仪检测其SERS光谱。结果傅里叶红外光谱仪检测修饰玻片在1 538 cm^-1处具有红外特征峰,紫外分光光度法测定修饰玻片万古霉素含量达0.02 mg/cm^2。在激光显微拉曼光谱仪633 nm激光下,检测出金黄色葡萄球菌在724 cm^-1、780 cm^-1、1 001 cm^-1、1 050 cm^-1、1 100 cm^-1、1 240 cm^-1具有SERS特征峰。结论万古霉素修饰玻片成功捕获金黄色葡萄球菌并检测出其SERS光谱,为直接从人体体液中捕获、鉴定金黄色葡萄球菌提供基础依据。
Objective To develop a method for Staphylococcus aureus detection based on surface-enhanced Raman spectroscopy( SERS). Methods By the catalysis of carbodiimide,vancomycin formed covalent binding with the aminated slide. The content of vancomycin coupled to the slide were measured by UV spectrophotometer. Using vancomycin-modified slide to capture and fix Staphylococcus aureus,the SERS spectrum of Staphylococcus aureus was detected by laser micro Raman spectrometer with the enhancement of silver nanoparticles. Results The characteristic infrared peak was detected by Fourier transform spectroscopy at 1 538 cm-1. The content of vancomycin in the modified slide reached to 0.02 mg/cm2 by UV spectrophotometer. Under laser of 633 nm SERS characteristic peaks of S. aureus was detectable at 724 cm-1,780 cm-1,1 001 cm-1,1 050 cm-1,1 100 cm-1 and 1 240 cm-1 by Raman micro-spectrometer. Conclusion The vancomycin-modified slide may be used to capture Staphylococcus aureus successfully and measured its SERS spectrum. The developed method in this study should provide a basis for direct capture and identification of Staphylococcus aureus in human body fluids.
作者
邰秋园
屈晓君
赵艳丰
柏婷婷
郭志睿
朱叶飞
TAI Qiuyuan;QU Xiaojun;ZHAO Yanfeng;BAI Tingting;GUO Zhirui;ZHU Yefei(Clinical Laboratory Medicine Center,the Second Affiliated Hospital of Nanjing Medical University,Nanjing 210011,Jiangsu;Department of Laboratory Medicine,Lishui District People's Hospital of Nanjing City,Nanjing 211200,Jiangsu,China)
出处
《临床检验杂志》
CAS
2020年第8期583-586,共4页
Chinese Journal of Clinical Laboratory Science
基金
国家自然科学基金青年基金(81802071)。
