摘要
目的了解2019年北京市某幼儿园聚集性发热疫情的病原分子生物学特征。方法采集北京市某幼儿园聚集性呼吸道发热疫情的病例咽拭子标本,利用实时荧光定量PCR方法对呼吸道病毒、肠道病毒和呼吸道细菌进行核酸筛检。对肠道病毒筛查阳性的标本利用肠道病毒VP1区特异性引物进行VP1片段扩增和测序,并对测序序列进行BLAST比对和进化关系分析。结果在收集的5例病例标本中,共检测到4例肠道病毒阳性标本,其他呼吸道病毒和呼吸道细菌检测均为阴性。VP1序列BLAST结果显示,4例肠道病毒均为柯萨奇病毒A6型(CVA6)。进化关系分析显示,该病毒与中国云南2018年的1株引起手足口病的CVA6毒株高度同源。结论北京市该起呼吸道聚集性发热疫情是由肠道病毒CVA6型引起。
Objective To understand the molecular biological characteristics of the pathogens of a clustered fever epidemic in a kindergarten in Beijing in 2019. Methods The throat swab specimens were collected from cases of clustered respiratory fever epidemic in a kindergarten in Beijing, and performed nucleic acid screening for respiratory viruses, enteroviruses and respiratory bacteria by real-time fluorescent quantitative PCR methods. The specimens with positive enterovirus screening were amplified and sequenced with VP1 fragments by specific primers of enterovirus VP1 region, and BLAST alignment and evolutionary relationship analysis were performed on the sequenced sequence.Results Among the 5 specimens collected, 4 specimens positive for enterovirus were detected, and the tests for other respiratory viruses and bacteria were negative. The BLAST results of VP1 sequence showed that the 4 cases of enterovirus were all Coxsackie virus A6(CVA6). Evolutionary relationship analysis showed that the virus is highly homologous to a CVA6 strain that caused hand, foot and mouth disease in Yunnan of China in 2018.Conclusion This respiratory cluster fever epidemic in Beijing is caused by enterovirus CVA6.
作者
李洪军
邹林
杨艳娜
郗璐
甄博珺
佟玲
张铁钢
张国峰
LI Hongjun;ZOU Lin;YANG Yan-na;XI Lun;ZHEN Bo-jun;TONG Ling;ZHANG Tie-gang;ZHANG Guofeng(Department of Infectious Disease and Endemic Disease Prevention and Control,Tongzhou District Center for Disease Control and Prevention,Beijing,101100,China;Laboralory,Tongzhou District Center for Disease Control and Prevention,Beijing,101100,China;Administration Office,Tongzhou District Center for Disease Control and Prevention,Beijing,101100,China;Institue of Immunization Prerention,Bejing Center for Disease Control and Prevenion,Beijing.100013.China)
出处
《职业与健康》
CAS
2020年第15期2123-2125,共3页
Occupation and Health
基金
国家科技重大专项-艾滋病和病毒性肝炎等重大传染病防治专项(2017ZX10103004)。
