摘要
工业上常用的芽孢杆菌为革兰氏阳性菌,被广泛作为异源蛋白表达的安全宿主系统(GRAS)。芽孢杆菌只有一层细胞外膜,蛋白分泌能力强,据资料报道,异源蛋白在芽孢杆菌表达系统中的表达量最高可以达到20 g/L以上,因此该系统非常适合表达分泌型异源蛋白。目前,已有多种芽孢杆菌作为表达宿主用于外源蛋白的过量表达,获得了很好的效果;并且实现了大量工业酶、药物蛋白等在芽孢杆菌表达系统中的规模化生产。本文对几种常见的芽孢杆菌表达宿主进行综述,讨论了现有芽孢杆菌作为表达系统的特点、应用及优缺点,并对其研究进行了初步展望,将为芽孢杆菌表达系统进一步研究及应用提供参考。
Bacillus expression system as industrial bacterial workhorse is generally regarded as safe(GRAS)for heterologous protein expression.Bacillus has only one membrane and strong protein secretion ability.According to reports,the expression of heterologous proteins in the Bacillus expression system can reach up to more than 20 g/L,so this system is very suitable for the expression of secretory.At present,a variety of Bacillus have been used as expression hosts for the heterogenous expression of target gene,and a good expression level has been obtained.Many industrial enzymes and recombinant pharmaceutical proteins have been successfully going into large scale production in the Bacillus expression system.This paper reviews the characteristics and application of several common Bacillus expression hosts,discusses the advantages and disadvantages of existing Bacillus expression system.It not only could give a preliminary outlook for its research,but also provide reference for further research and application of Bacillus expression system.
作者
张心怡
沈镇炎
段绪果
李慧星
Zhang Xinyi;Shen Zhenyan;Duan Xuguo;Li Huixing(Department of Food Science and Technology,College of Light Industry and Food Engineering,Nanjing Forestry University,Nanjing,210037;Henan Key Laboratory of Industrial Microbial Resources and Fermentation Technology,Nanyang,473000)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2020年第7期3110-3118,共9页
Genomics and Applied Biology
基金
国家自然科学基金(31401636)
南京林业大学青年拔尖人才基金项目(GXL2018010)
河南省工业微生物资源与发酵技术重点实验室开放课题(IMRFT20180102)共同资助。
关键词
芽孢杆菌
表达系统
研究进展
Bacillus
Expression system
Research progress
