摘要
为解析番茄斑萎病毒(TSWV)长距离运动机制,利用透射电子显微镜观察TSWV在系统寄主普通烟草K326维管组织各类细胞中的分布特征。结果表明:导管内有囊泡包裹的病毒粒体及病毒核壳体聚集体(NCA)类似物;维管薄壁细胞内发现病毒粒体分布于细胞质的内质网池中,细胞质中有囊泡增多现象;伴细胞中发现有成熟的病毒粒体,并发现大量直径120 nm左右的囊泡小体,伴随有线粒体的基质膨大,线粒体崩解等现象;筛管中未发现有病毒粒体,但筛管中发现有较多直径80~200 nm的囊泡小体。提示TSWV病毒粒体有可能通过导管进行长距离运动,TSWV囊泡相关复合体(VRC)有可能通过筛管进行长距离运动。
In this study,the distribution of tomato spotted wilt virus(TSWV)in vascular tissues of systemic host tobacco K326 was observed by transmission electron microscopy(TEM)to analyze the long-distance transportation of TSWV.The results showed that vesicle-enveloped viral particles and nuclear capsid aggregates(NCA)were found in vessel elements.Vesicle-enveloped viral particles were found in vascular parenchyma cells,and there were more vesicles in cytoplasm than the CK.Vesicle-enveloped viral particles were found in companion cells,and a large number of vesicles with a diameter of about 120 nm were found in cytoplasm.At the same time,pathological changes in mitochondria were also found,including the expansion and disappearance of the mitochondrial stroma lamella.There was no viral particles in the sieve element,but vesicles with a diameter of about 80-200 nm were found in the sieve element.Our finding suggested that the TSWV virions are likely transported by xylem,and the TSWV vesicle-related complexes(VRC)may be transported by phloem.
作者
温志多
丁勇
尚卫娜
王田田
吴少政
苏晓霞
张洁
张仲凯
郑宽瑜
Wen Zhiduo;Ding Yong;Shang Weina;Wang Tiantian;Wu Shaozheng;Su Xiaoxia;Zhang Jie;Zhang Zhongkai;Zheng Kuanyu(Key Laboratory of Forest Biotechnology in Yunnan,Southwest Forestry University,Kunming Yunnan 650233,China;Biotechnology and Germplasm Resources Institute,Yunnan Academy of Agricultural Sciences,Kunming Yunnan 650205,China;Life Sciences Institute,Zhejiang University,Hangzhou Zhejiang 310058,China)
出处
《西南林业大学学报（自然科学）》
CAS
北大核心
2020年第6期124-131,共8页
Journal of Southwest Forestry University：Natural Sciences
基金
国家自然科学基金项目(31660508,31560499)资助
云南省基础研究计划面上项目(2018FB028)资助
国家自然科学基金-云南联合基金重点项目(U1802235)资助
云南省云岭学者培养计划项目资助。
关键词
番茄斑萎病毒
长距离运动
维管组织
透射电子显微镜
组织分布
tomato spotted wilt virus
long-distance transportation
vascular tissue
transmission electron microscopy
tissue distribution
