摘要
目的:以血清脂质浓度、抗氧化损伤、肝脏损伤以及脂肪酸合成酶的水平为观测指标,探究桦褐孔菌多糖(Inonotus obliquus polysaccharide,IOP)对高脂血症大鼠肝脏疾病的干预作用。方法:将40只雄性SD大鼠随机分为正常对照组、高脂模型组和桦褐孔菌多糖低、中、高剂量组。采用试剂盒测定血脂指标:总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白(high-density lipoprotein,HDL)、低密度脂蛋白(low-density lipoprotein,LDL)水平;抗氧化指标:超氧化物歧化酶(superoxide diamutase,SOD)活力、丙二醛(malondialdehyde,MDA)含量、谷胱甘肽过氧化物酶(glutathion peroxide,GSH-Px)活力;肝损伤指标:谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(asparate aminotransferase,AST);脂肪酸合成酶(fatty acidsynthetase,FAS)、羟甲基戊二酰辅酶A还原酶(human hydroxymethylglutaryl CoA reductase,HMGCR)活力。结果:相较于高脂模型组,高剂量(200 mg/kg mb)的桦褐孔菌多糖对于降低大鼠血脂水平,保护肝脏受损有明显的作用效果,其中TC、TG、LDL水平明显下降,HDL水平明显上升,MDA浓度降低,SOD、GSH-Px活力升高,FAS和HMGCR活力明显降低。结论:桦褐孔菌多糖能够对高脂饮食引起的高脂血症大鼠脂质紊乱、肝脏损伤起到保护作用,可能与其抗氧化作用有关。
Objective:To investigate the intervention effect of Inonotus obliquus polysaccharide(IOP)on liver disease in hyperlipidemic rats by measuring serum lipid content,antioxidant defense indices,liver damage and fatty acid synthase activity.Methods:Forty male Sprague-Dawley(SD)rats were randomly divided into normal control,hyperlipidemia model and low-,medium-and high-dose IOP groups.Commercial kits were employed to determine the following blood lipid parameters:total cholesterol(TC),triglyceride(TG),high-density lipoprotein(HDL),low-density lipoprotein(LDL);antioxidant indices:superoxide diamutase(SOD),malondialdehyde(MDA)and glutathion peroxide(GSH-Px);liver injury indices:alanine aminotransferase(ALT)and aspartate aminotransferase(AST);and fatty acid synthetase(FAS)and human hydroxymethylglutaryl CoA reductase(HMGCR).Results:Compared with the high-fat group,high-dose(200 mg/kg mb)of Inonotus obliquus polysaccharide has a significant effect on reducing blood lipid levels in rats and protecting liver from damage.And the dose group TC,TG,LDL decreased significantly,HDL increased significantly,MDA content decreased,SOD,GSH-Px activity increased,FAS and HMGCR activity decreased significantly.Conclusion:Polysaccharide from Inonotus obliquus can protect against lipid disorders,liver damage in rats with hyperlipidemia caused by high-fat diet,may be related to its antioxidant effect.
作者
崔敬爱
王思霁
刘畅
于婷
陈晓平
CUI Jingai;WANG Siji;LIU Chang;YU Ting;CHEN Xiaoping(College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2020年第19期185-190,共6页
Food Science
基金
吉林省重点科技攻关项目(20170204006YY)。
关键词
桦褐孔菌
多糖
降血脂
肝损伤
脂质过氧化
Inonotus obliquus
polysaccharide
hypolipidemic
liver injury
lipid peroxidation
