摘要
目的:探讨芎麻汤不同提取物对新生SD大鼠三叉神经节神经元瞬时受体电位香草酸亚型1(TRPV1)的影响。方法:新生SD大鼠三叉神经节神经元分离、原代培养,应用TRPV1拮抗剂及芎麻汤不同提取物(石油醚部位、乙酸乙酯部位、正丁醇部位、萃取后水溶液部位)进行干预,采用RT-qPCR、Western Blot方法检测各组神经元TRPV1 mRNA、蛋白的表达情况。结果:①与空白对照组比较,芎麻汤石油醚提取物(1×10^-6mg/mL)及萃取后水提物(1×10^-4mg/mL)能显著降低正常三叉神经节神经元TRPV1 mRNA表达(P<0.05)。②与空白对照组比较,TRPV1拮抗剂能显著升高正常神经元TRPV1蛋白表达(P<0.01)。与TRPV1拮抗剂对照组比较,芎麻汤石油醚提取物(1×10^-3、1×10^-4、1×10^-7mg/mL)、乙酸乙酯提取物(1×10^-3、1×10^-4、1×10^-6、1×10^-7mg/mL)、正丁醇提取物(1×10^-3、1×10^-4mg/mL)、萃取后水提物(1×10^-3mg/mL)能显著降低拮抗剂干扰的神经元TRPV1蛋白表达(P<0.01,P<0.05)。结论:芎麻汤不同提取物能下调新生大鼠三叉神经节神经元TRPV1 mRNA、蛋白表达。
Objective:To investigate the different extracts of Xiongma Decoction on TRPV1 in trigeminal ganglion neurons of neonatal rat.Methods:The trigeminal ganglion from newly born 3 d SD rats were taken to obtain trigeminal ganglion neurons by using typeⅠcollagenase and trypsin digestion,and primary cultured neurons.After treatment of trigeminal ganglion neurons with TRPV1 antagonist and different extracts of Xiongma Decoction,RT-qPCR and Western Blot were used to detect the expression of TRPV1 mRNA and protein in each group.Results:①Compared with the blank control group,the petroleum ether extract of Xiongma Decoction(1×10^-6 mg/mL)and the aqueous extract after extraction(1×10^-4 mg/mL)can significantly reduced the expression of TRPV1 mRNA in normal trigeminal ganglion neurons(P<0.05).②Compared with the blank control group,TRPV1 antagonist can significantly increase the expression of TRPV1 protein in normal neurons(P<0.01).Compared with the TRPV1 antagonist group,the petroleum ether extract of Xiongma Decoction(1×10^-3,1×10^-4,1×10^-7 mg/mL),the ethyl acetate extract(1×10^-3,1×10^-4,1×10^-6,1×10^-7 mg/mL),the n-butanol extract(1×10^-3,1×10^-4 mg/mL)and the extracted water extract(1×10^-3 mg/mL)can significantly reduce the expression of TRPV1 protein in the neurons interfered with by the TRPV1 antagonist(P<0.01,P<0.05).Conclusion:The extract of Xiongma Decoction can down-regulated the expression of TRPV1 mRNA and protein in trigeminal ganglion neurons of neonatal rat.
作者
刘顶鼎
刘冬
周英
郭建生
杨菁
曾贵荣
LIU Ding-ding;LIU Dong;ZHOU Ying;GUO Jian-sheng;YANG Jing;ZENG Gui-rong(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Beijing 100050,China;Hunan University of Chinese Medicine,Changsha 410208,China;Hunan Key Laboratory of Pharmacodynamics and Safety Evaluation of New Drugs&Hunan Provincial Research Center for Safety Evaluation of Drugs,Changsha 410331,China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2020年第9期4380-4385,共6页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(No.81860844)
贵州省科学技术基金计划项目(No.黔科合基础[2016]1016,No.黔科合基础[2019]1038号)
贵州省教育厅青年科技人才成长项目(No.黔教合KY字[2016]185)
贵州省中医药、民族医药科学技术研究专项(No.QZYY-2016-001)
天然药物活性物质与功能国家重点实验室开放课题(No.GTZK201609)。
