摘要
【目的】解析洱海流域油菜-水稻轮作农田土壤细菌多样性特征,探讨水旱轮作对土壤微生物多样性的影响。【方法】运用微生物核糖体RNA基因靶向测序技术和荧光定量PCR技术,研究了连续7Y油菜-水稻种植后土壤细菌种群结构的组成、丰度和碳氮转化功能基因丰度变化特征,结合Perason相关性分析,解析细菌种群结构与土壤碳氮素之间的关系。【结果】土壤有机质(OM)和全氮(TN)与细菌种群中的变形菌门(Proteobacteria)和酸杆菌门(Acidobacteria)的种群相对丰度呈显著负相关关系,与绿弯菌门(Chloroflexi)和放线菌门(Actinobacteria)的种群相对丰度呈显著正相关关系。在油菜和水稻种植季,不同处理间Clean tags数量、OTUs丰度及α多样性指数差异性不显著(P≥5),门分类水平上的优势细菌种群相对丰度差异性不显著(P≥5)。土壤碳氮转化功能基因qPCR统计分析结果表明,处理间固碳基因cbbL-R和氨氧化基因amoA的拷贝数差异性不显著,有机碳源物料配施化肥显著提高固氮基因nifH和氨氧化基因amoA拷贝数(P<0.05)。【结论】油菜和水稻种植季土壤细菌种群结构组成和碳氮转化功能基因丰度呈相似变化趋势,水旱轮作对土壤细菌种群多样性的影响不显著,其稳定性可能与土壤本底较高的碳氮素储量有关。
【Objective】The purpose of this study was to analyze the characteristics of soil bacterial diversity in rape-rice rotation farmland in Erhai basin,so as to further explore the impact of water and drought rotation on soil microbial diversity.【Method】Using microbial ribosomal RNA gene targeting sequencing technology and fluorescence quantitative PCR technology,the change characteristics of soil bacterial population abundance and carbon-nitrogen conversion function gene abundance were studied.Further,the relationship between bacterial diversity,population structure and soil carbon,nitrogen was analyzed combined with Perason correlation analysis.【Result】The results showed that soil organic matter(OM)and total nitrogen(TN)were negatively correlated with the relative abundance of Proteobacteria and Acidobacteria,and positively correlated with the relative abundance of Chloroflexi and Actinobacteria.For rape and rice planting seasons,the number of clean tags,the abundance of OTUs and the diversity index between different treatments were not significant(P≥5),and the dominant bacterial populations at the phylum level was not significant(P≥5).The qPCR results showed that there was no significant difference between treatments of the copies of cbbl-R gene and amoA,while the copies number of nifH and amoA gene were significantly increased by applying organic carbon source materials(P<0.05).【Conclusion】The changes of soil bacterial diversity,population structure and carbon and nitrogen conversion gene abundance were similar in rape and rice planting seasons.The effect of crop rotation on soil bacterial diversity was not significant,and its stability might be related to the soil background of higher carbon and nitrogen content.
作者
伏成秀
李铭刚
吴翔
付斌
张庆
施竹凤
倪明
胡万里
杨明英
朱红业
杨佩文
FU Cheng-xiu;LI Ming-gang;WU Xiang;FU Bin;ZHANG Qing;SHI Zhu-feng;NI Ming;HU Wan-li;YANG Ming-ying;ZHU Hong-ye;YANG Pei-wen(Institute of Agricultural Environmental Resources,Yunnan Academy of Agricultural Sciences,Yunnan Kunming 650205,China;Yunnan University,Yunnan Kunming 650091,China;Institute of Soil and Fertilizer,Sichuan Academy of Agricultural Sciences,Sichuan Chengdu 610066,China)
出处
《西南农业学报》
CSCD
北大核心
2020年第9期1898-1904,共7页
Southwest China Journal of Agricultural Sciences
基金
国家自然科学基金(31660600,31760019)
农业农村部西南山地农业环境重点实验室开放基金(AESMA-OPP-2019001)。
关键词
油菜-水稻轮作
土壤细菌种群结构
高通量测序
碳氮转化功能基因
qPCR定量分析
Rape-rice rotation
Soil microbial population
High-throughput sequencing
Carbon and nitrogen conversion function gene
qPCR quantitative analysis
