摘要
本研究对甜瓜根部病原菌进行分离纯化,并进行ITS序列扩增及测序、系统进化树构建、致病力测定。结果表明,供试菌株为番茄匍柄霉,其可对甜瓜叶片造成17.2 mm的病斑;番茄匍柄霉(Stemphylium lycopersici)有可能是甜瓜潜在的病原菌。
In this paper, the pathogens from the roots of Cucumis melo L. were isolated and purified. ITS sequence amplification and detection, phylogenetic tree construction, and pathogenicity determination were carried out. The results showed that the tested strain was identified as Stemphylium lycopersici, which could cause 17.2 mm lesions on leaves of Cucumis melo L.;Stemphylium lycopersici might be a potential pathogen of Cucumis melo L.
作者
郝芳敏
臧全宇
马二磊
丁伟红
黄芸萍
王毓洪
HAO Fangmin;ZANG Quanyu;MA Erlei;DING Weihong;HUANG Yunping;WANG Yuhong(Ningbo Academy of Agricultural Sciences,Ningbo Zhejiang 315040;Ningbo Key Laboratory of Melon and Vegetable Breeding,Ningbo Zhejiang 315040)
出处
《现代农业科技》
2020年第20期89-90,95,共3页
Modern Agricultural Science and Technology
基金
宁波市“科技创新2025”重大专项“优质多抗西甜瓜特异种质创制及新品种选育与推广”(2019B10007)。
关键词
甜瓜
病原菌
番茄匍柄霉
分离纯化
致病力测定
Cucumis melo L.
pathogen
Stemphylium lycopersici
isolation and purification
pathogenicity determination
