摘要
为了获得潜在高产2,3-丁二醇的酿酒酵母菌株,探究不同菌株生产2,3-丁二醇的潜力。通过对不同供试菌株进行形态观察、产物耐受性检测、关键酶活测定、乙偶姻产量以及发酵试验,筛选出一株生产2,3-丁二醇最有潜力的菌株酿酒酵母(Saccharomyces cerevisiae)W141。结果表明,发现W141菌株生长迅速,能分别耐受13%(w/v)乙醇和0.8%(w/v)乙酸,具有较高的乙醇得率(0.593 g/g)和碳源利用率(发酵24 h葡萄糖消耗率为100%),关键酶α-ALS、BDH的酶活力较高,分别是0.046 U/mg和0.040 U/mg,2,3-丁二醇途径关键中间产物乙偶姻含量较高,可达0.246 g/L。说明该菌株在发酵工业中具有一定的改造空间和应用前景。
The aims are to obtain the potentially high-production 2,3-butanediol Saccharomyces cerevisiae, and study the 2,3-butanediol producing potential of different strains. The most promising Saccharomyces cerevisiae strain W141 for producing 2,3-butanediol was screened by morphological observation, product tolerance detection, key enzyme activity assay, acetoin yield and fermentation test. The results showed that the W141 grew rapidly, could withstand 13%(w/v) ethanol and 0.8%(w/v) acetic acid, respectively, and had the high ethanol production(0.593 g/g) and carbon source utilization rate(the glucose consumption rate was 100% after fermentation for 24 h). The enzyme activities of key enzymes α-ALS and BDH were higher, 0.046 U/mg and0.040 U/mg, respectively. The key intermediate of the 2,3-butanediol pathway, acetoin, had a high content,which reached 0.246 g/L. The study indicates that Saccharomyces cerevisiae W141 has certain transformation space and application prospect in the fermentation industry.
作者
丁昊
刘文娟
孙健
刘磊
平文祥
葛菁萍
Ding Hao;Liu Wenjuan;Sun Jian;Liu Lei;Ping Wenxiang;Ge Jingping(Key Laboratory of Microbiology of Heilongjiang Province,Life Science College,Heilongjiang University,Harbin 150080;Agricultural Microorganisms Technology Education Engineering Research Center,Harbin 150500)
出处
《中国农学通报》
2020年第24期107-115,共9页
Chinese Agricultural Science Bulletin
基金
黑龙江省自然科学基金重点项目“乙酸代谢与副干酪乳杆菌群体感应互作探究及对细菌素产生的影响”
国家自然科学基金“从2,3-丁二醇代谢角度构建工程微生物群体及其生态学机制研究”(31570492)
黑龙江省教育厅重点项目“利用肺炎克雷伯氏菌发酵生产2,3-丁二醇及机理探讨”(HDJCCX-2016Z05)。
关键词
2
3-丁二醇
酿酒酵母
菌株筛选
发酵
关键酶活力
菌株代谢产物
2
3-butanediol
Saccharomyces cerevisiae
strain screening
fermentation
key enzyme activity
strain metabolite
