摘要
为从定性和定量2个方面比较宽叶山蒿与艾的化学成分差异,该文以不同种源的艾与宽叶山蒿的叶和绒为研究对象,采用UPLC-Q-TOF-MS技术鉴定了样品中18个化合物的结构,利用主成分分析(PCA)和正交偏最小二乘法判别分析(OPLS-DA)筛选差异标志物,并利用UPLC-UV同时测定了12个化学成分的含量。结果表明宽叶山蒿与艾所含主要成分类似,主要为绿原酸类和黄酮类,除个别成分的有无区别外主要是含量高低上具有差异。艾中的高车前素未在宽叶山蒿中检测到,夏佛塔苷、异绿原酸B和异绿原酸C为艾叶与宽叶山蒿叶的差异性化合物,异绿原酸A、异绿原酸C和棕矢车菊素为艾绒与宽叶山蒿绒的差异性化合物。艾中棕矢车菊素和夏佛塔苷含量存在明显差异。另外,宽叶山蒿野生比栽培的异绿原酸A含量高。该方法成功评价了宽叶山蒿与四大名艾的整体质量,为宽叶山蒿的下一步开发利用提供了科学依据。
The chemical component information of samples was analyzed by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS).The leaves and flosses of Artemisia argyi and A.stolonifera from different places,were distinguished by principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA).Then,compounds with significant differences(P<0.01)in abundance were screened out according to their VIP values and t-test.Eighteen components in all samples have been filtered and identified,including flavonoids and chlorogenic acids,the content of the 12 of which were measured by UPLC-UV,which are different in presence and content.Hispidulin in A.argyi is not detected in A.stolonifera.Schaftaside,isochlorogenic acid B,and isochlorogenic acid C are differential compounds of A.argyi and A.stolonifera leaves.Isochlorogenic acid A,isochlorogenic acid C and jaceosidin are differential compounds of A.argyi and A.stolonifera floss.There are significant differences in the contents of jaceosidin and schaftoside in the four famous A.argyi.In addition,the content of isochlorogenic acid A in wild A.stolonifera is higher than that in cultivated A.stolonifera.The results of the study successfully clarified the differences between A.stolonifera and A.argyi,and provided theoretical and data references for the further development and utilization of A.stolonifera.
作者
罗丹丹
彭华胜
张元
黄显章
詹志来
刘大会
康利平
黄璐琦
LUO Dan-dan;PENG Hua-sheng;ZHANG Yuan;HUANG Xian-zhang;ZHAN Zhi-lai;LIU Da-hui;KANG Li-ping;HUANG Lu-qi(Pharmacy Faculty,Huibei University of Chinese Medicine,Wuhan 430065,China;Pharmacy Faculty,Anhui University of Chinese Medicine,Hefei 230012,China;State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Henan Key Laboratory of Zhang Zhongjing Formulae and Herbs for Immunoregulation,Nanyang Institute of Technology,Nanyang 473004,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2020年第17期4057-4064,共8页
China Journal of Chinese Materia Medica
基金
中央本级重大增减支项目(2060302)
国家重点基础研究发展计划(973)项目(2015CB554506)
国家重点研发计划项目(2017FYC1700704)
现代农业产业技术体系建设专项(CARS-21)。
