摘要
文章选用母本东农46和父本L-100构建重组自交群体127个家系为试验材料,利用全基因组重测序技术构建高密度遗传连锁图谱,结合群体豆荚长、宽、厚性状R3~R8时期表型数据,利用QTL IciMapping 4.1.0.0软件完备区间作图法分析非条件和条件QTL加性和上位性效应。结果表明,在豆荚3个性状发育过程中,共检测到40个非条件加性效应QTL,贡献率为4.35%~25.52%;检测到12个条件加性效应QTL,贡献率为3.80%~23.32%。同时检测到多个QTL位点在不同性状或不同生育时期表达,如QPL-7-1、QPL-12-2和QPL-20-1(QPW-20-1)等。通过上位性分析检测到豆荚长度143对、宽度185对和厚度34对QTL位点,贡献率最高分别达29.69%、24.73%和25.17%,表明多基因效应在豆荚生长发育过程中发挥重要作用。
In this study,total 127 lines of recombinant inbred lines population from female parent Dongnong 46 and male parent L-100 were selected as materials,and a high-density genetic linkage map was constructed using the whole-genome resequencing technology.The inclusive complete interval mapping method of QTL IciMapping 4.1.0.0 software was used to analysis additive and epistatic effects of conditional and non-conditional QTL of pod length,pod width and pod thickness.The results showed that during the development of the three traits of the pod,a total of 40 non-conditional additive QTLs were detected with a contribution rate of 4.35%-25.52%,12 QTLs with a conditional additive effect were detected,with a contribution rate of 3.80%-23.32%.At the same time,many non-condition and condition QTLs were detected simultaneously in different traits or different periods,such as QPL-7-1,QPL-12-2 and QPL-20-1(QPW-20-1).Total 143 pairs QTL of length,185 pairs QTL of width and 34 pairs QTL of thickness were acquired by the epistasis analys,with the highest contribution rates reaching 29.69%,24.73%and 25.17%,respectively,which indicated that the polygenic effects played an important role in the growth and development of seed pods.
作者
滕卫丽
郭志文
郑立娜
付雪
王博
董莹莹
张丹洋
刘赫禹
冯文婧
赵雪
韩英鹏
李文滨
TENG Weili;GUO Zhiwen;ZHENG Lina;FU Xue;WANG Bo;DONG Yingying;ZHANG Danyang;LIU Heyu;FENG Wenjing;ZHAO Xue;HAN Yingpeng;LI Wenbin(Institute of Soybean Research,Key Laboratory of Soybean Biology in Chinese of Ministry Education,Key Laboratory of Soybean Biology and Breeding(Genetics)of Chinese Agriculture and Rural Ministry,Northeast Agricultural University,Harbin 150030,China)
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2020年第10期1-9,共9页
Journal of Northeast Agricultural University
基金
黑龙江省“百千万”工程科技重大专项(2019ZX16B01-1)
国家自然科学基金项目(31471517)
现代农业产业技术体系建设专项资金项目(CARS-04-PS04)
国家十三五重点研发计划项目(2017YFD0101306-05)。
