摘要
目的:观察鲜茵陈提取物对人肝癌HepG2细胞株增殖的抑制和凋亡的诱导作用。方法:采用MTT法观察鲜茵陈提取物对HepG2增殖的抑制作用,Annexin V-FITC/PI染色流式细胞仪检测鲜茵陈提取物对细胞凋亡的诱导。结果:不同浓度的提取物在不同时间内对HepG2的增殖有抑制作用,随着提取物浓度的增加和作用时间的延长,抑制增殖的作用越来越明显,凋亡逐渐增加。结论:鲜茵陈提取物对HepG2有明显的增殖抑制的作用,能够促进细胞的凋亡。
Objective:To observe the inhibition of proliferation and the induction of apoptosis of extract of fresh Artemisia capillaris Thunb.on human hepatocellular carcinoma cell line HepG2.Methods:The inhibitory effect of the extract of fresh Artemisia capillaris Thunb.on the proliferation of HepG2 was observed by MTT method,and the apoptosis induction effect was detected by Annexin V-FITC/PI staining.Results:Different concentrations of extract could inhibit the proliferation of HepG2 at different intervention time.Moreover,with the increase of extract concentration and prolonged action time,the effect of inhibiting proliferation became more and more obvious,and apoptosis increased gradually.Conclusion:The extract of fresh Artemisia capillaris Thunb.can obviously inhibit the proliferation of HepG2 cells and promote the apoptosis of the cells.
作者
袁满
张君利
傅风华
吕剑涛
YUAN Man;ZHANG Junli;FU Fenghua;LYU Jiantao(School of Pharmacy,Yantai University,Yantai 264005,China;Yantai Affiliated Hospital of Binzhou Medical University,Yantai 264005,China)
出处
《中医药信息》
2020年第6期45-49,共5页
Information on Traditional Chinese Medicine
基金
山东省中医药科技发展计划项目(2017-236)
滨州医学院科研计划与科研启动基金(BY2017KJ42)
滨州医学院科研启动基金(BY2018KYQD12)。
关键词
鲜茵陈提取物
HEPG2
增殖
凋亡
Extract of fresh Artemisia capillaris Thunb.
HepG2
Proliferation
Apoptosis
