摘要
目的检测并研究GP6基因rs1654419位点、纤维蛋白原(Fg)Bβ-455G/A(FgBβ-455G/A)基因的多态性与深静脉血栓形成(DVT)患者发病的相关性。方法采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)的方法对2016年4月至2019年11月之间在我院接受治疗的100例DVT患者以及同一时间段内在我院体检中心接受体检的100例健康体检者的基因型进行分析。其中对照组为健康体检者,观察组为确诊DVT的患者,均记录血浆Fg浓度、D-Dimer水平。结果在观察组与对照组中,FgBβ-455位点处基因型为GG、AG、AA的血浆中Fg浓度水平依次增高,差异具有统计学意义(P<0.05);而GP6基因rs1654419位点处基因型为GG、AG、AA的血浆中D-Dimer浓度水平也依次增高,差异具有统计学意义(P<0.05);FgBβ-455位点和GP6基因rs1654419位点的A等位基因在观察组中频率高于对照组中频率,差异具有统计学意义(P<0.05)。结论FgBβ-455-AA、GP6基因rs1654419位点-AA使机体形成DVT的风险显著提高,是DVT发病的独立危险因素。
Objective To detect and study the relationship between the polymorphism of GP6gene rs1654419locus and fibrinogen Bβ-455G/A(FgBβ-455G/A)gene and the pathogenesis of deep venous thrombosis(DVT).Methods Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)was used to analyze the genotypes of 100patients with DVT(observation group)treated in our hospital and the healthy control(control group)in the physical examination center of our hospital between April 2016and November 2019.The plasma Fg concentration and D-Dimer level were recorded in both groups.Results In the observation group and the control group,the plasma Fg concentration of GG,AG and AA increased successively at the site of FgBβ-455,and this result showed a significant difference(P<0.05);and the levels of D-Dimer has the same trend at GP6gene rs1654419locus,this result also showed a significant difference(P<0.05).The frequency of A allele at FgBβ-455locus and GP6gene rs1654419locus in the observation group was significantly higher than that in the control group(P<0.05).Conclusion FgBβ-455-AA and GP6gene rs1654419locus-AA can significantly increase the risk of DVT,and they can be independent risk factor for the pathogenesis of DVT.
作者
耿力
孙冰
高波
陈琰
GENG Li;SUN Bing;GAO Bo(The Second Hospital of Jilin University,Changchun 130041,China)
出处
《中国实验诊断学》
2020年第9期1424-1428,共5页
Chinese Journal of Laboratory Diagnosis
基金
吉林省科技发展计划项目(20190201092JC)。
