摘要
目的:研究黄芪甲苷(Astragaloside Ⅳ,AS-Ⅳ)干预高糖受损人内皮祖细胞(Endothelial Progenitor Cells,EPCs)增殖的最佳浓度,并探讨AS-Ⅳ对高糖受损EPCs生物学功能的影响。方法:取足月新生儿脐带血分离、培养并鉴定EPCs,将鉴定成功的EPCs用30 mmol/L的葡萄糖预处理120 h后,随机分为实验组(HG+AS-Ⅳ组)和对照组(HG组),同时设置正常组(NC组)。用CCK8检测不同浓度AS-Ⅳ(0、25、50、100、200、400 mg/L)干预EPCs 24 h后的增殖情况,绘制增殖曲线,初步得出AS-Ⅳ干预高糖受损人EPCs增殖的最佳浓度。进而通过CCK-8增殖实验、黏附能力测定试验、细胞划痕试验及Matrigel体外成血管实验检测最佳浓度的AS-Ⅳ对高糖受损EPCs增殖、黏附、迁移和成管功能的影响。结果:AS-Ⅳ促高糖受损EPCs增殖的最佳浓度为100 mg/L;与对照组比较,100 mg/L AS-Ⅳ干预后的高糖受损EPCs增殖能力、黏附细胞数、细胞迁移率、体外成管数均明显增加,差异均有统计学意义(P1<0.05);同时检测实验组与正常组差异无统计学意义(P2>0.05)。结论:AS-Ⅳ可显著改善体外高糖受损的人EPCs的生物学功能,恢复其原有活力并具有介导血管新生的潜能。
Objective:To study the optimal concentration of Astragaloside Ⅳ(AS-Ⅳ)on the proliferation of human endothelial progenitor cells(EPCs)impaired by high glucose,and to explore the biological effects of AS-Ⅳ on high glucose impaired EPCs.Methods:Full-term neonatal umbilical cord blood was isolated,cultured,and EPCs were identified.The identified EPCs were pretreated with 30 mmol/L glucose for 120 hours,and were randomly divided into an experimental group(HG+AS-Ⅳ group)and a control group(HG Group),while the normal group(NC group)was set.CCK8 was used to detect the proliferation of EPCs at different concentrations of AS-Ⅳ(0,25,50,100,200,400 mg/L)after 24 hours,and the proliferation curve was drawn to obtain the preliminary conclusion that AS-Ⅳ interfered with the proliferation of EPCs in patients with high glucose damage.Optimal concentration.Furthermore,the effects of the optimal concentration of AS-Ⅳ on the proliferation,adhesion,migration,and tube-forming function of high-glucose-damaged EPCs were tested by CCK-8 proliferation assay,adhesion ability test,cell scratch test,and Matrigel in vitro angiogenesis experiment.Results:The optimal concentration of AS-Ⅳ to promote the proliferation of high-glucose-damaged EPCs was 100 mg/L;compared with the control group,100 mg/L AS-Ⅳ-treated high-glucose-damaged EPCs had the proliferation capacity,the number of adherent cells,and cell migration The rate and the number of tube formation in vitro were significantly increased,and the differences were statistically significant(P1<0.05).At the same time,there was no significant difference between the experimental group and the normal group(P2>0.05).Conclusion:AS-Ⅳ can significantly improve the biological function of human EPCs with impaired high glucose in vitro,restore their original vitality and have the potential to mediate angiogenesis.
作者
余亦程
熊武
蔡昫
王禹萌
肖慧
白雪
邹晓玲
YU Yicheng;XIONG Wu;CAI Xu;WANG Yumeng;XIAO Hui;BAI Xue;ZOU Xiaoling(Hunan University of Chinese Medicine,Changsha 410208,China;The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China)
出处
《世界中医药》
CAS
2020年第21期3244-3249,共6页
World Chinese Medicine
基金
国家自然科学基金青年科学基金项目(81904217)
湖南省卫生计生委科研计划课题项目(B20180739)。
关键词
黄芪甲苷
内皮祖细胞
生物学功能
体外实验
Astragaloside Ⅳ
Endothelial progenitor cells
Biological function
In vitro experiment
