摘要
玉米芯是一种重要的木质纤维素生物质农业废弃物,因其降解困难、利用率较低而未得到充分利用。从寒地黑土中筛选出一株高效降解木质纤维素的放线菌GS-3-39,对其进行生物学形态鉴定及16S rRNA分子生物学鉴定,判定GS-3-39属于链霉菌属。采用单因素结合响应曲面法对其发酵条件进行优化,并对降解前后的玉米芯进行红外和电镜观察。结果表明:当培养基初始pH值为5.06、发酵温度28.22℃、发酵转速163.98 r/min、接菌量为3.09%时,玉米芯木质纤维素降解率理论最优值为26.881%,重复试验得到降解率为27.264%。在此条件下对玉米芯进行降解,结果表明,放线菌GS-3-39对玉米芯木质纤维素具有降解作用。
Corn cob is an important agricultural waste of lignocellulosic biomass,which contains a large amount of cellulose,hemicellulose and lignin.However,because of its degradation difficulty,it is not fully utilized,resulting in resource waste.A high efficient degradation of cellulose actinomycetes strains GS-3-39 was selected from the cold region and black glebe,its biological morphology and molecular biology of 16S rRNA were identified,the single factor experiment was done,significantly higher factors were selected by Plackett Burman,its fermentation conditions were optimized by response surface method(RSM),scanning electron microscope(SEM)and Fourier IR characterization were determined before and after degradation of corn cob.The results showed that GS-3-39 was identified as streptomyces after morphological observation and molecular biological identification.After optimization by RSM,when the initial pH value of the medium was 5.06,the fermentation temperature was 28.22℃,the fermentation revolution was 163.98 r/min,and the inoculated-pathogen quantities was 3.09%,the maximum degradation rate of GS-3-39 pair of corn cob was 27.264%,and the degradation rates of cellulose,hemicellulose and lignin in the corn cob were 39.84%,38.33%and 47.38%,respectively.The results of SEM and IR on corn cob before and after degradation showed that actinomycetes GS-3-39 had significant degradation effect on corn cob cellulose.
作者
刘晓飞
侯艳
马京求
关桦楠
张娜
马永强
LIU Xiaofei;HOU Yan;MA Jingqiu;GUAN Hua'nan;ZHANG Na;MA Yongqiang(School of Food Engineering,Harbin University of Commerce,Harbin 150076,China)
出处
《农业机械学报》
EI
CAS
CSCD
北大核心
2020年第11期329-337,共9页
Transactions of the Chinese Society for Agricultural Machinery
基金
黑龙江省“百千万”工程科技重大专项(2019ZX08B02)。
关键词
玉米芯
纤维素
放线菌
降解
响应曲面优化
corn cob
cellulose
actinomycetes
degradation
response surface methodology
