摘要
利用介孔纳米材料富集信号探针,采用T-2毒素适配体作为识别探针和信号探针的控制开关,检测体系中的靶标T-2毒素可以与识别探针T-2毒素适配体进行特异性结合并释放出信号探针,根据信号强度与靶标毒素含量的相关关系建立一种非标记型T-2毒素快速定量检测技术。结果表明,应用本法对玉米、大麦、小麦等样品检测加标回收率为85.8%~111.4%,相对标准偏差在4.6%以内,具有良好的检测准确性和重复性;同时该方法灵敏度高,方法检出限为1.25 ng/mL,且在不同污染范围内(0~50、50~250 ng/mL)线性相关性较强,与传统的实验方法相比,该方法具有快速、简便、廉价等优点,可在较宽的线性范围内进行T-2毒素的高灵敏度定量检测。
In this paper,using mesoporous silica nanoparticles(MSN)to enrich the signal probe and using the T-2 toxin aptamer as a recognition probe and a switch to control the signal probe,a rapid label-free technique for the quantitative detection of T-2 toxin was proposed based on the relationship between the signal intensity and the content of the target toxin.In the detection system,the target T-2 toxin could specifically bind to its aptamer,releasing the signal probe.The results showed that when this method was applied to corn,barley and wheat samples,the spiked recoveries of T-2 toxin were in the range between 85.8%and 111.4%with relative standard deviation(RSD)less than 4.6%,indicating that this method has good accuracy and repeatability.The method was sensitive with a limit of detection of 1.25 ng/mL and showed a good linearity in the concentration range of 0-50 and 50-250 ng/mL.Compared with the traditional method,this novel method was more rapid,simpler and cheaper and allowed sensitive and quantitative detection of T-2 toxin with a wider linear range.
作者
徐群博
谭红霞
马良
XU Qunbo;TAN Hongxia;MA Liang(College of Food Science,Southwest University,Chongqing 400715,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2020年第22期324-329,共6页
Food Science
基金
国家级大学生创新创业训练计划项目(201910635029)
重庆市技术创新与应用示范(社会民生类)项目(cstc2018jscx-msybX0204)。
关键词
T-2毒素
适配体
介孔二氧化硅纳米颗粒
非标记
荧光
T-2 toxin
aptamer
mesoporous silica nanoparticles
label-free
fluorescence spectroscopy
