摘要
目的:探讨红景天苷对急性心肌梗死(AMI)大鼠心肌相关凋亡因子及B淋巴细胞瘤-2基因(Bcl-2)、Bcl-2相关X蛋白(Bax)、半胱氨酸天冬氨酸蛋白酶-3 (Caspase-3)表达的影响。方法:75只健康SPF级SD大鼠,随机选择10只作为假手术组,仅进行开胸、缝合处理;剩余65只采用冠脉结扎诱导建立大鼠AMI模型,剔除不合格模型后共有50只标准大鼠AMI模型,随机分为模型组、阳性对照组及红景天苷低、中、高剂量组,每组10只。阳性对照组按10 mg/kg的剂量给予卡维地洛;红景天苷低、中、高剂量组分别按12、24、36 mg/kg的剂量给予红景天苷,均溶于10 mL生理盐水灌胃给药;假手术组和模型组给予等量生理盐水灌胃。模型制备成功后次日开始灌胃,持续8周。原位末端凋亡检测(TUNEL)法检测心肌细胞凋亡情况,苏木精-伊红(HE)染色法测定梗死面积,BCA法测定Bcl-2、Bax、Caspase-3蛋白表达,荧光定量PCR法检测Bcl-2、Bax、Caspase-3 m RNA相对表达。结果:与假手术组比较,模型组大鼠心肌细胞凋亡指数(AI)、心肌梗死面积、Bax、Caspase-3蛋白及mRNA相对表达均增加(P<0.05),Bcl-2蛋白及mRNA相对表达均降低(P<0.05)。与模型组比较,阳性对照组及红景天苷低、中、高剂量组大鼠心肌细胞AI、心肌梗死面积、Bax、Caspase-3蛋白及mRNA相对表达均降低(P<0.05),Bcl-2蛋白及m RNA相对表达均升高(P<0.05)。与阳性对照组比较,红景天苷低、中剂量组大鼠心肌细胞AI、心肌梗死面积、Bax、Caspase-3蛋白及m RNA相对表达升高(P<0.05);Bcl-2蛋白及mRNA相对表达降低(P<0.05)。结论:红景天苷可通过下调Bax、Caspase-3的表达,上调Bcl-2的表达,来减少细胞凋亡,缩小大鼠AMI模型梗死面积。
Objective: To investigate the effect of salidroside on the expression of myocardial-associated apoptotic factors and levels of B-cell lymphoma-2(Bcl-2),Bcl-2-associated X protein(Bax) and aspartic acid-specific protease-3(Caspase-3) in rats with acute myocardial infarction(AMI). Methods:A total of 75 healthy SPF-level SD rats were randomly selected, and 10 of them were randomly selected as the sham operation group, which were only performed with thoracotomy and suture;the remaining 65 rats were induced by coronary ligation to establish AMI rat models. After the rejection of unqualified models, a total of 50 standard AMI rat models were randomly divided into the model group, the positive control group,and the low,medium and high dose salidroside groups,with 10 rats in each group. The positive control group was given carvedilol at 10 mg/kg. The low,medium and high dose salidroside groups were given 12,24 and 36 mg/kg of salidroside respectively,which were all soluble in 10 m L of normal saline. The sham operation group and the model group were given the same amount of normal saline by gavage. The next day after the successful preparation of the models,the gavage began and lasted for eight weeks. The terminal deoxynucleotidyl transferase d UTP nick end labeling(TUNEL) was used to detect the apoptosis of cardiomyocytes,the hematoxylin-Eosin staining(HE) for infarct area,BCA staining for protein expressions of Bcl-2,Bax and Caspase-3,and fluorescence quantitative PCR for m RNA expressions of Bcl-2,Bax and Caspase-3. Results:Compared with those in the sham operation group,the apoptosis index(AI),myocardial infarct area,protein and m RNA expressions of Bax and Caspase-3 in the model group were increased(P<0.05), and the protein and m RNA expressions of Bcl-2 were decreased(P<0.05). Compared with those in the model group,the AI,myocardial infarct area,protein and m RNA expressions of Bax and Caspase-3 in the positive control group and the low,medium and high dose salidroside groups were all decreased(P<0.05),and the protein and m RNA expressions of Bcl-2 were increased(P<0.05).Compared with those in the positive control group,the AI,myocardial infarct area,protein and m RNA expressions of Bax and Caspase-3 in the low and medium dose salidroside groups were increased(P<0.05), and the protein and m RNA expressions of Bcl-2 were decreased(P<0.05). Conclusion:Salidroside can reduce the apoptosis of cells and the infarct area of AMI rats by down-regulating the expression of Bax and Caspase-3 and up-regulating the expression of Bcl-2.
作者
廖骏
陆建忠
王巍
LIAO Jun;LU Jianzhong;WANG Wei
出处
《新中医》
CAS
2020年第21期5-9,共5页
New Chinese Medicine
基金
浙江省基础重点专科项目(ZD20192017786)。
