摘要
该研究基于黄瓜基因组数据库,利用生物信息学和荧光定量PCR等方法,对黄瓜SOS2基因家族进行全基因组鉴定,并对其表达特性进行系统分析。结果表明:(1)在黄瓜基因组中,共鉴定到15个SOS2基因(CsSOS2-1~CsSOS2-15),且不均匀分布在5条染色体上;亚细胞定位显示SOS2蛋白主要位于细胞质膜。(2)系统进化分析显示,CsSOS2-2和CsSOS2-6与AtSOS2亲缘关系更近。(3)顺式作用元件预测显示,SOS2基因启动子序列主要含有干旱诱导和防御应激响应元件。(4)结构分析显示,SOS2蛋白所含保守基序的排列顺序完全一致,且主要含有STKc和NAF保守结构域,这些结构可能在基因响应盐胁迫过程中起调控作用。(5)荧光定量试验表明,SOS2基因家族主要在黄瓜的根和叶片响应盐胁迫时上调表达,其中,盐胁迫处理1 d时有5个基因上调表达,并随处理时间延长盐胁迫下的基因表达有所下调;增施多胺显著上调了CsSOS2-1~CsSOS2-5、CsSOS2-8、CsSOS2-9、CsSOS2-12和CsSOS2-15在不同组织中的表达,说明盐胁迫下多胺能诱导黄瓜SOS2基因家族的表达,进而参与植物耐盐分子网络的调控。
The SOS signal transduction pathway is a salt stress signal response pathway in plants,and serine/threonine protein kinase(SOS2)plays an important role in this pathway.Based on the cucumber genome database,this study used bioinformatics and quantitative real-time PCR and other methods to identify the whole genome of cucumber SOS2 gene family,and systematically analyzed its expression characteristics.The results showed that:(1)a total of 15 SOS2 genes(CsSOS2-1-CsSOS2-15)were identified in the cucumber genome,which were distributed unevenly on 5 chromosomes,and the subcellular localization showed that SOS2 protein were mainly located in the plasma membrane.(2)Phylogenetic analysis showed that CsSOS2-2 and CsSOS2-6 were more closely related to AtSOS2.(3)The prediction of cis-acting elements shows that the promoter sequence of SOS2 gene mainly contains drought-induced and defense-stress response elements.(4)Structural analysis showed that the sequence of conserved motifs contained in SOS2 protein were completely consistent,and mainly contained STKc and NAF conserved domains,which may play a regulatory role in gene response to salt stress.(5)Quantitative real-time PCR test showed that the SOS2 gene family is mainly upregulated in cucumber roots and leaves in response to salt stress.Among them,6 genes were up-regulated in response to salt stress on the first day of treatment.As the treatment time prolonged,gene expression was down-regulated under salt stress.Addition of polyamines significantly increased the expression of CsSOS2-1—CsSOS2-5,CsSOS2-8,CsSOS2-9,CsSOS2-12 and CsSOS2-15 in different tissues.This shows that polyamines can induce the expression of cucumber SOS2 gene family under salt stress,and then participate in the regulation of plant salt tolerance molecular network.
作者
王丹怡
韩玲娟
张毅
张莉
刘东旭
李斌
WANG Danyi;HAN Lingjuan;ZHANG Yi;ZHANG Li;LIU Dongxu;LI Bin(Collaborative Innovation Center for Improving Quality and Increase Profits of Protected Vegetables, College of Horticulture, Shanxi Agricultural University, Taigu, Shanxi 030801, China;College of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi 030801, China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2020年第11期1855-1865,共11页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金(31501806)
山西农业大学博士科研启动项目(2014YJ14,2020BQ20)
山西省重点研发计划重点项目子课题(201703D211001-04-03)。
