摘要
背景:干细胞来源外泌体携带有助于细胞间信号传导的miRNA、蛋白质等生物活性物质,对神经修复过程有着积极影响,具有类似于干细胞移植的治疗作用,可用于代替干细胞移植治疗周围神经损伤。目的:总结干细胞来源外泌体在周围神经损伤治疗中的应用效果,为外泌体治疗周围神经损伤提供参考及依据。方法:检索PubMed数据库中2000至2020年的相关文章,英文检索词为“peripheral nerve,injury,damage,stem cells,embryonic stem cells,induced pluripotent stem cells,mesenchymal stem cells,exosomes,repair,regenerate”,检索中国知网、维普、万方等数据库2000至2020年的相关文章,中文检索词为“周围神经,损伤,损害,干细胞,胚胎干细胞,诱导多能干细胞,间充质干细胞,外泌体,再生,修复”,对文献资料及参考文献进行逐一查阅。结果与结论:干细胞来源外泌体具有与干细胞类似的组织修复再生和保护能力,可避免干细胞移植时出现的免疫排斥、致瘤风险等问题,且具有更好的细胞外基质结合能力,是进行“无细胞”治疗的新型策略,但是干细胞来源外泌体应用于周围神经修复仍然有许多基础性研究有待积累,与组织工程和基因工程的结合需进一步探索。
BACKGROUND:Stem cell-derived exosomes carry biologically active substances such as miRNAs and proteins that contribute to intercellular signal transduction,which have an important and positive impact on the nerve repair.They have a therapeutic effect similar to stem cell transplantation and can be used to replace stem cell transplantation for treating peripheral nerve injury.OBJECTIVE:To summarize the application of exosomes from stem cells in the treatment of peripheral nerve injury,so as to provide reference and basis for exosomes applied in the treatment of peripheral nerve injury.METHODS:Articles in PubMed database from 2000 to 2020 were searched using the search terms of“peripheral nerve,injury,damage,stem cells,embryonic stem cells,induced pluripotent stem cells,mesenchymal stem cells,exosomes,repair,regenerate.”Articles in databases of CNKI,VIP and WANFANG from 2000 to 2020 were retrieved with the search terms of“peripheral nerve,injury,damage,stem cells,embryonic stem cells,induced pluripotent stem cells,mesenchymal stem cells,exosomes,repair,regenerate.”The literature and references were reviewed one by one.RESULTS AND CONCLUSION:Stem cell-derived exosomes exert powerful tissue repair,regeneration and protection capabilities similar to those of stem cells.Stem cell-derived exosomes are a new strategy for“cell-free”therapy,because they can avoid immune rejection,tumorigenic risks and other problems during treatment with stem cell transplantation and have better capabilities of binding extracellular matrix.However,the application of stem cell-derived exosomes in peripheral nerve research still needs a lot of basic researches to be accumulated,and the combination with tissue engineering and genetic engineering needs further exploration.
作者
王康
智晓东
王伟
Wang Kang;Zhi Xiaodong;Wang Wei(Department of Orthopedics,First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000,Liaoning Province,China;Institute of Orthopedics,Jinzhou Medical University,Jinzhou 121000,Liaoning Province,China;Liaoning Key Laboratory of Medical Tissue Engineering,Jinzhou 121000,Liaoning Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2021年第19期3083-3089,共7页
Chinese Journal of Tissue Engineering Research
基金
辽宁省教育厅科学技术研究地方服务项目(JYTFUDF201757),项目负责人:王伟
锦州医科大学校企合作基金项目(2020002),项目负责人:王伟
辽宁省科学技术基金项目(20180551216),项目负责人:智晓东。
关键词
干细胞
间充质干细胞
外泌体
周围神经损伤
组织工程
基因工程
再生
综述
stem cells
mesenchymal stem cells
exosomes
peripheral nerve injury
tissue engineering
genetic engineering
regeneration
review
