摘要
该文拟在前期复方甘草微乳研究基础上,制备甘草微乳凝胶剂,并研究其对慢性湿疹的治疗效果。采用单因素法优选凝胶基质的种类、用量、加入方式及保湿剂用量,确定甘草微乳凝胶剂的处方工艺;以甘草酸、甘草苷和氧化苦参碱为评价指标,采用Franz扩散池法测定复方甘草微乳凝胶的体外释放行为;选择小鼠迟发型超敏反应的湿疹模型为药效评价模型,测定耳肿胀率、耳片炎性细胞浸润程度、耳片组织病理改变,考察对小鼠慢性湿疹的治疗效果。复方甘草微乳凝胶剂制备工艺稳定,甘草苷和氧化苦参碱的释放最符合Hixcon-Crowell动力学模型,而甘草酸的释放最符合Ritger-Peppas动力学模型;药效学研究证明,复方甘草微乳凝胶剂可以显著降低小鼠耳肿胀率,抗炎效果较好,具有对抗慢性湿疹病理改变及抑制真皮炎性细胞浸润的作用。因此,复方甘草微凝胶制备工艺可行,释药稳定,对慢性湿疹具有显著治疗效果。
Based on the previous study of compound liquorice microemulsion, this paper aims to prepare the compound liquorice microemulsion gel and investigate its pharmacodynamics of chronic eczema. The type, dosage and adding method of gel matrix, and formula dosage of humectant were optimized by single factor method to obtain the formula and preparation technique of the gel. With glycyrrhizic acid, glycyrrhetin and oxymatrine used as evaluation indexes, the Franz diffusion cell method was adopted to monitor the in vitro release profile of the gel. Eczema model of delayed-type hypersensitivity in mice was chosen to detect the ear swelling rate, degree of inflammatory cell infiltration of ear pieces, and pathological changes of ear pieces, so as to investigate the therapeutic effect of the microemulsion gel. The preparation process of the compound liquorice microemulsion gel was stable. The release of glycyrrhizin and oxymatrine was most consistent with the Hixcon-Crowell kinetic model, while the release of glycyrrhizic acid was most consistent with the Ritger-Peppas kinetic model. The pharmacodynamics studies proved that compound liquorice microemulsion gel could significantly reduce the ear swelling rate in mice, with good anti-inflammatory effect as well as the ability to resist the pathological changes of chronic eczema and inhibit the infiltration of dermal inflammatory cells. Therefore, the preparation process of compound liquorice microemulsion gel is feasible, with stable drug release and a significant therapeutic effect on chronic eczema.
作者
王景雁
马书伟
赵馨雨
陈佳佳
刘玉娟
邓莉莉
郭子右
吴清
WANG Jing-yan;MA Shu-wei;ZHAO Xin-yu;CHEN Jia-jia;LIU Yu-juan;DENG Li-li;GUO Zi-you;WU Qing(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China;Shandong Dyne Marine Biopharmaceutical Co.,Ltd.,Weihai 264300,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2020年第21期5193-5199,共7页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81473365)
北京市自然科学基金项目(7192109)。
关键词
微乳凝胶剂
体外释放
经皮给药
慢性湿疹
microemulsion gel
in vitro release
transdermal delivery
chronic eczema
