摘要
本文建立了一种超高效液相色谱-串联质谱法快速检测大豆中多菌灵药物残留量的分析方法。样品采用乙腈进行提取,经正己烷净化,通过多反应检测模式测定,外标法定量测定。实验结果表明,多菌灵在质量浓度0.5~50μg/L范围内具有良好的线性系数,线性方程为y=7523.32x+786.168,相关系数为0.9993,方法的检出限和定量检出限分别为0.0029μg/kg和0.0097μg/kg。进行加标回收试验,添加多菌灵质量浓度水平分别为1、5、10μg/L,加标回收率分别为88.2%、92.4%和95.1%,相对标准偏差小于6.26%。本方法具有快速、简便、灵敏度高等优点,能适用于大豆中多菌灵的残留量检测。
The ultra high performance liquid chromatography-tandem mass spectrometry method was developed for the rapid detection of carbendazim drug residues in soybean. The samples were extracted with acetonitrile, purified by n-hexane, determined by multiple reaction detection mode and quantified by external standard method. The results show that the linear coefficient of carbendazim is good in the range of 0.5-50 μg/L with a correlation at 0.9993, and the detection limit and limit of quantification are 0.0029μg/kg and 0.0097μg/kg, respectively. The recovery rates of the samples with the concentration of 1, 5, 10 μg/L are 88.2%, 92.4% and 95.1% respectively, with relative standard deviation(RSD) below 6.26 %. The method is fast, simple, and highly sensitive for determination of carbendazim in soybean.
作者
李文敏
张健玲
柳映青
谢思瑶
蒋小良
LI Wen-Min;ZHANG Jian-Ling;LIU Ying-Qing;XIE Si-Yao;JIANG Xiao-Liang(Technology Center of Jiangmen Customs,Jiangmen 529000;China Certification&Inspection and Group Guangdong Co.,Ltd,Guangzhou 510000)
出处
《中国口岸科学技术》
2020年第11期20-24,共5页
China Port Science and Technology
关键词
超高效液相色谱-串联质谱法
多菌灵
大豆
ultra high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS)
carbendazim
soybean
