摘要
通过单因素和正交试验对多糖提取工艺中的料液比、提取时间、提取温度等进行优化,并以维生素C(Vc)为阳性对照,对滇黄精多糖进行抗氧化活性研究.结果表明,在最佳提取工艺即料液比为1∶20,提取时间为2 h,提取温度为80℃时,滇黄精多糖提取率达13.34%,并且在一定浓度下,滇黄精多糖对DPPH自由基、羟基自由基以及超氧阴离子自由基有不同程度清除作用,具有一定的抗氧化活性.
In this research, the effects of the material-liquid ratio, the extraction time and extraction temperature in the extraction process of polysaccharide from Polygonatum kingianum were investigated by the single-factor and orthogonal tests. And the antioxidant activity of polysaccharide was studied by using vitamin C(Vc) as a positive control. The results showed that the best extraction conditions of polysaccharide from Polygonatum kingianum were as follows: the material-liquid ratio was 1∶20, the extraction time was 2 h, and the extraction temperature was 80 ℃. Under the best extraction conditions, the extraction rate of polysaccharide was 13.34%. And polysaccharide from Polygonatum kingianum showed some ability to scavenge DPPH free radicals, hydroxyl free radicals and superoxide anion free radicals to varying degrees, and had certain antioxidant activity.
作者
李智敏
石瑶
赵纯希
于丽娟
张易莲
张庭
李晚谊
肖丹
LI Zhi-mi;SHI Yao;ZHAO Chun-xi;YU Li-juan;ZHANG Yi-lian;ZHANG Ting;LI Wan-yi;XIAO Dan(Institute of Medicinal Plants,Yunnan Academy of Agricultural Sciences,Kunming 650223,China;Institute of Agro-Products Processing,Yunnan Academy of Agricultural Sciences,Kunming 650221,China;College of Tropical Crop Science,Yunnan Agricultural University,Pu'er 665099,China)
出处
《云南民族大学学报（自然科学版）》
CAS
2020年第6期535-540,共6页
Journal of Yunnan Minzu University:Natural Sciences Edition
基金
云南省重点研发计划(农业领域)(2018BB024)
云南省重大科技专项(2017AB004)。
关键词
滇黄精多糖
提取工艺
抗氧化活性
polysaccharide from Polygonatum kingianum
extraction
antioxidation activity
