摘要
【目的】观察凤尾草提取物抗腹泻的主要药效。【方法】通过70%乙醇回流提取,60℃旋转蒸发获得凤尾草提取物。小鼠随机分组,各组预防性给药后,观察凤尾草提取物对正常小鼠胃排空、小肠推进的影响,对多潘立酮导致小鼠小肠运动亢进的影响,对小鼠腹腔毛细血管通透性的影响,对蓖麻油所致腹泻的影响。【结果】正常小鼠的小肠运动试验中,单药凤尾草的作用在雌雄之间有差异,5 g/kg凤尾草乙醇提取物连续给药3 d可显著抑制(P<0.05)雌性小鼠胃排空,极显著(P<0.01)抑制小肠运动,而显著(P<0.05)促进雄性小鼠胃排空及小肠运动;多潘立酮负荷下小鼠的小肠推进试验中,5 g/kg凤尾草乙醇提取物能拮抗多潘立酮,显著(P<0.05)抑制雄性小鼠小肠推进,对雌性小鼠的小肠推进抑制作用无显著差异(P>0.05);冰醋酸致小鼠腹腔毛细管通透性试验中,与模型组相比较,5 g/kg凤尾草乙醇提取物极显著(P<0.01)降低雄性小鼠腹腔毛细管通透性,抑制率达37.327%;凤尾草抗蓖麻油致小鼠腹泻试验中,在观察的5 h内,凤尾草乙醇提取物有抗腹泻作用,凤尾草提取物高剂量组疗效比低剂量组好。凤尾草高剂量组发挥止泻的起效时间在2 h,主要疗效的时间段在2~5 h;与模型组相比,凤尾草低剂量组在0~3 h的平均稀便级显著降低(P<0.05),凤尾草高剂量组在0~2 h的平均稀便级显著降低(P<0.05),0~3 h、0~4 h极显著降低(P<0.01);表明蓖麻油致小鼠腹泻模型试验中,凤尾草提取物有很好的止泻作用,且高剂量组比低剂量组作用更强。【结论】凤尾草具有抗腹泻作用。
[Objective]To observe the main anti-diarrhea effect of the extract of Pteris multifda Poir.[Method]The extracts were obtained by refluxing 70%ethanol and rotating evaporation at 60℃.Mice were randomly divided into groups.After preventive administrationin to each group,the effects of the extract of Pteris multifda Poir.on gastric emptying and small intestine propulsion,hyperactivity of small intestine caused by domperidone,permeability of capillaries in abdominal cavity and diarrhea caused by castor oil were observed.[Result]In the intestinal motility test of normal mice,there was a difference between male and female.5 g/kg ethanol extract of Pteris multifda Poir.could significantly inhibit(P<0.05)the gastric emptying of female mice,extremely significantly(P<0.01)inhibit the intestinal motility of male mice,and significantly(P<0.05)inhibit the gastric emptying and intestinal motility of male mice.In the intestinal propulsive test of mice under the loading of domperidone,5 g/kg ethanol extract of Pteris multifda Poir.could antagonize domperidone,significantly(P<0.05)inhibited the intestinal propulsive effect of male mice,but had no significant difference on the intestinal propulsive effect of female mice(P>0.05).In the experiment of capillary permeability of abdominal cavity in mice induced by glacial acetic acid,compared with the model group,5 g/kg ethanol extract of Pteris multifda Poir.significantly reduced the capillary permeability of the abdominal cavity of male mice,with the inhibition rate of 37.327%.In the experiment of anti diarrhea of mice induced by castor oil,the ethanol extract of Pteris multifda Poir.had the effect of anti diarrhea within 5 hours.The high dose extract Pteris multifda Poir.group had better effect than the low dose group.In the high dose group,the onset time of antidiarrheal effect was 2 hours,and the main effect time was 2-5 hours.Compared with the model group,the average loose stool level of low dose group decreased significantly(P<0.05)at 0-3 h,the average loose stool level of high dose group decreased significantly(P<0.05)at 0-2 h,and extremely significantly decreased at 0~3 h and 0~4 h(P<0.01).The results showed that in the diarrhea model test of mice induced by castor oil,the extract of Pteris multifda Poir.had a good antidiarrheal effect,and the effect of high dose group was stronger than that of low dose group[.Conclusion]Pteris multifda Poir.has the effect of anti-diarrhea.
作者
南苹瑶
王立琦
鲍光明
郭小权
王小莺
NAN Ping-yao;WANG Li-qi;BAO Guang-ming;GUO Xiao-quan;WANG Xiao-ying(Institute of Veterinary Medicine,College of Institute of Animal Science and Technology Jiangxi Agricultural University,Nanchang 330045,China;weinan Animal Disease Prevention and Control Center,Weinan,Shanxi714000,China)
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2020年第6期1222-1230,共9页
Acta Agriculturae Universitatis Jiangxiensis
基金
江西省重点研发计划项目(20161BBF60088)。
关键词
凤尾草
胃排空
小肠推进
肠运动亢进
蓖麻油
腹腔通透性
Pteris multifda Poir.
gastric emptying
small intestine propulsion
hypermotility of intestine
castor oil
abdominal permeability
