摘要
目的:探究结直肠癌血清生长分化因子-15(GDF-15)、粒细胞集落刺激因子(G-CSF)水平与患者临床病理参数、预后的关系,并分析其对结直肠癌的诊断价值。方法:采集结直肠癌患者、结直肠腺瘤患者及健康体检志愿者的血清样本,酶联免疫吸附测定(ELISA)实验检测血清GDF-15、G-CSF;分析结直肠癌患者血清GDF-15、G-CSF水平与患者临床病理参数的关系;Kaplan Meier生存曲线分析血清GDF-15、G-CSF水平与患者预后的关系;受试者工作特征曲线(ROC)分析血清GDF-15、G-CSF水平对结直肠癌的诊断价值。结果:结直肠癌患者血清GDF-15、G-CSF水平高于结直肠腺瘤患者及健康体检志愿者(均P<0.05);血清GDF-15水平与肿瘤直径、分化程度、远处转移及TNM分期相关(均P<0.05);血清G-CSF水平与肿瘤分化程度、远处转移及TNM分期相关(均P<0.05);Kaplan Meier生存曲线结果显示,血清GDF-15低表达、G-CSF低表达患者的术后5年总生存率及中位生存时间均分别高于血清GDF-15高表达、G-CSF高表达患者(均P<0.05);ROC分析结果表明,血清GDF-15、G-CSF有较好的诊断结直肠癌的价值,血清GDF-15、G-CSF联合应用的敏感度、特异度分别为0.876、0.863。结论:结直肠癌患者血清GDF-15、G-CSF水平升高,且均与患者病情恶性进展、不良预后相关;血清GDF-15、G-CSF是诊断结直肠癌的潜在指标。
Objective: To investigate the correlation of serum growth differentiation factor-15(GDF-15), granulocyte colony stimulating factor(G-CSF) levels with the clinicopathological characteristics and prognosis in patients with colorectal cancer, and to analyze their diagnostic value for colorectal cancer. Methods: Serum samples were collected from patients with colorectal cancer, patients with colorectal adenoma and healthy volunteers. The serum levels of GDF-15, G-CSF were detected by the enzyme linked immunosorbent assay(ELISA). The correlation between the serum GDF-15, G-CSF with the clinicopathological characteristics were analyzed. Kaplan Meier survival curve was used to analyse of the relationship between serum GDF-15, G-CSF levels and prognosis of patients. The diagnostic value of serum GDF-15 and G-CSF levels in colorectal cancer was analyzed by receiver operating characteristic curve(ROC). Results: The serum levels of GDF-15 and G-CSF in patients with colorectal cancer were higher than those in patients with colorectal adenoma and healthy volunteers(all P<0.05). Serum GDF-15 level were related with tumor diameter, differentiation degree,distant metastasis and the TNM stage(all P<0.05). Serum G-CSF level were related with differentiation degree, distant metastasis and the TNM stage(all P<0.05). Kaplan Meier results showed that the 5-year overall survival rate, the medium survival time in the low GDF-15 group and the low G-CSF group were higher than those in the high GDF-15 group and the high G-CSF group(all P<0.05). Moreover,ROC analysis showed that serum GDF-15, G-CSF had a good value in the diagnosis of colorectal cancer, the sensitivity and specificity of combined detection of serum GDF-15, G-CSF were 0.876 and 0.863 respectively. Conclusion: The serum GDF-15, G-CSF in the colorectal cancer patients are up-regulated, which are both related with malignant progression and poor prognosis. The serum GDF-15,G-CSF are potential indicators for the diagnosis of colorectal cancer.
作者
杨建华
王静
牛广旭
田云霄
刘增宾
郝增方
YANG Jian-hua;WANG Jing;NIU Guang-xu;TIAN Yun-xiao;LIU Zeng-bin;HAO Zeng-fang(Department of Pathology,Handan Central Hospital of Hebei Province,Handan,Hebei,056001,China;Department of Eastern District Laboratory,Second Hospital of Hebei Medical University,Shijiazhuang,Hebei,050000,China;Department of Pathology,Second Hospital of Hebei Medical University,Shijiazhuang,Hebei,050000,China)
出处
《现代生物医学进展》
CAS
2020年第20期3992-3996,共5页
Progress in Modern Biomedicine
基金
河北省医学科学研究计划项目(20190515)。
