摘要
目的通过比较实验兔和猫对组胺标准品的降压反应,探讨实验兔应用于药品降压物质检查的可行性。方法取普通级的新西兰兔、日本大耳白兔、青紫蓝兔和猫各6只,雌雄各半,经戊巴比妥钠麻醉后通过静脉分别注射0.05~5.0μg/kg(实验兔)、0.05~1.0μg/kg(猫)的组胺标准品溶液以及0.5 m L/kg和1.0 m L/kg的生脉注射液。首先通过比较3种实验兔对组胺标准品降压反应的敏感性、重现性和血压稳定性等指标,再比较实验兔和猫对组胺灵敏度试验结果与《中国药典》要求的符合性及其对组胺标准品与生脉注射液降压反应的差异性。结果3种实验兔对组胺标准品降压反应的敏感性和重现性均比较一致,品种间没有显著性差异,但在实验过程中新西兰兔血压较为稳定且操作方便;与猫相比较,实验兔对组胺降压反应极不敏感,其降压反应值约为猫的1/8,且其灵敏度试验结果不符合药典的要求,但对生脉注射液却和猫一样具有明显的降压反应,降压值约为猫2/3。结论实验兔对组胺物质的降压反应不敏感,暂时无法用于降压物质检查,但对生脉注射液仍较敏感,说明组胺对动物的降压作用具有明显的动物种属选择性。
Objective To explore the feasibility of appling the laboratory rabbits in the test for depressor substances of drugs by comparing the differences of depressor response to histamine between the laboratory rabbits and cats.Method The amount of conventional New Zealand rabbits,Japanese white rabbits,Chinchilla rabbits and healthy cats was respectively 6,half male and half female.After anesthetized with pentobarbital sodium,they were injected intravenously with standard histamine solution by a dose of 0.05—5.0μg/kg(rabbits)or 0.05—1.0μg/kg(cats)and Shengmai injection both by a dose of 0.5 m L/kg and 1.0 m L/kg.We try firstly to compare the sensitivity,reproducibility and stability of blood pressure of the depressor responses to the standard histamine solution among three stocks of laboratory rabbits.Then the consistency of the result in the sensitivity test for histamine with the requirement of Chinese Pharmacopoeia(ChP)was also compared,and whether was different for histamine and Shengmai injection that induce depressor effects between the laboratory rabbits and cats.Result The sensitivity and reproducibility of the depressor response to histamine in 3 stocks of rabbits were consistent,without any significant differences,but it was easier to inject by the ear margin veins of New Zealand rabbits,with more stable blood pressure during operating.Comparing with cats,the rabbits were not very sensitive to the depressor effect of histamine,its depressor response was only about 1/8 of cats,so that the result of histamine sensitivity test did not meet the requirement of ChP.However,Shengmai injection also had the same significant depressor response to all the laboratory rabbits as the cats,which was about 2/3 of that of the cats.Conclusion The laboratory rabbits are not sensitive to histamine and not be currently used for the test for depressor substances,but as being sensitive to Shengmai injection,so that the study also demonstrated that histamine had obviously species selectivity on the depressor response of animals.
作者
黎家敏
马佳丽
杨春
邓林
马文静
荣祖元
LI Jiamin;MA Jiali;YANG Chun;DENG Lin;MA Wenjing;RONG Zuyuan(Sichuan Institute for Food and Drug Control,Chengdu 611731,China)
出处
《实验动物科学》
2020年第5期1-8,共8页
Laboratory Animal Science
基金
四川省食品药品检验检测院院立项目(No.2018-KYYL-007)。
关键词
实验兔
猫
降压物质检查
laboratory rabbits
cats
test for depressor substances
