摘要
目的:建立快速、准确测定人血浆中利奈唑胺浓度的方法,并应用于临床。方法:建立UPLC法,以苯妥英钠为内标,色谱柱采用Agilent ZORBAX SB-C18柱(100 mm×2.1 mm,1.8μm),柱温30℃;流动相为甲醇∶水=55∶45(V∶V),等度洗脱,流速0.3 ml/min,检测波长254 nm,进样量20μl。将此方法应用于血液科患者血浆中利奈唑胺浓度的测定。结果:利奈唑胺的线性范围为0.105~21.00μg/ml,最低定量限为0.105μg/ml(信噪比>5)。利奈唑胺的相对回收率均>85%,低、中、高浓度质控样品的日内、日间RSD均<15.0%。结论:该测定方法精密、准确,能快速测定人血浆中利奈唑胺的浓度,适用于临床利奈唑胺血药浓度监测和药动学研究,有利于利奈唑胺个体化用药方案的调整。
Objective:To establish a fast and accurate method to determine the concentration of linezolid in human plasma.Methods:The UPLC method was established with phenytoin sodium as internal standard and Agilent ZORBAX SB-C18 column as separate column.The mobile phase was methanol and water(55∶45,V∶V)with a flow rate of 0.3 ml/min.The detective wavelength was set at 254 nm and the injection volume was 20μl.The established method was used to monitor the concentration of linezolid in the plasma of the patients from the Department of Hematology.Results:The linear range of linezolid was 0.105-21.00μg/ml,with a minimum quantitative limit of 0.105μg/ml(S/N>5).The relative recovery rate of linezolid was>85%,and the RSD of the inter-day and intra-day precisions at low,medium and high concentrations were all<15%.Conclusion:The method is sensitive and accurate for the determination of linezolid in human plasma,and could be used for clinical drug monitoring and pharmacokinetic research of linezolid.Moreover,it is beneficial to the adjustment of individual medication regimen of linezolid.
作者
陈明
沈越凡
范国荣
唐原君
CHEN Ming;SHEN Yuefan;FAN Guorong;TANG Yuanjun(Department of Clinical Pharmacy,Shanghai General Hospital,Shanghai Jiao Tong University,Shanghai 200080,China;Department of Pharmacy,Jiangqiao Hospital,Jiading District of Shanghai,Shanghai 201800,China)
出处
《药学服务与研究》
CAS
2020年第6期409-413,共5页
Pharmaceutical Care and Research
基金
上海市卫生计生系统重要薄弱学科建设计划资助项目(2016ZB0302)。
关键词
利奈唑胺
血药浓度
色谱法
高效液相
linezolid
blood concentration
chromatography,high performance liquid
