摘要
以高光效小麦品种百农4199为材料,利用不同浓度NaCl模拟盐分胁迫对BN4199在不同胁迫时间点的幼苗和根系形态指标、叶片渗透性调节和抗氧化反应进行研究.结果表明:低浓度的盐分不影响或促进小麦幼苗生长,较高浓度的盐胁迫对幼苗生长产生一定抑制作用.无盐和低盐浓度(浓度为50 mmol/L NaCl)下的小麦幼苗干质量、苗相对生长率和根苗长度比高于其它浓度,根系干质量对高盐浓度具有一定的耐受力.幼苗脯氨酸在高浓度盐分下含量较高,可溶性蛋白含量没有随浓度变化表现出规律性,随着胁迫时间延长,脯氨酸和可溶性蛋白的增长率在高盐浓度下相对较大.高盐浓度(浓度为150和200 mmol/L)下,SOD活性始终保持较高,POD活性随浓度的增加先升高后降低;各种抗氧化酶活性变化率在不同浓度下表现出差异性.低盐浓度的NaCl促进根系总根长、总投影面积和总根表面积的增加,提高了对水分和养分的吸收,根长径级.主要分布在0.15<RD≤0.30 mm区间.高浓度NaCl抑制总根长和总表面积的增加,0.30<RD≤0.45 mm区间的细根分布是高盐浓度下幼苗根系的主要组成部分.
Bainong 4199 with high photosynthetic rate wheat seedlings was used to investigate the effects of NaCl stress with different concentrations on seedling and root morphological,osmotic adjustment substances and antioxidation responses in the leaves of wheat seedlings at different times.The results showed that low concentration NaCl stress did not affect or enhanced the growth of the seedling.High concentration NaCl stress inhibited seedling growth to a certain extent.The seedlings dry weight,seedling relative growth rate and the root shoot length ratio were higher in 0 and 50 mmol/L NaCl than other concentration NaCl stress.Root dry weight has tolerance to stress under high concentration of salt.Proline content of seedling was increased at high concentrations salinity stress.The soluble protein contents didn't show regularity with salt concentration changed.With the stress time increasing,the growth rate of proline and soluble protein content were relatively high under the high salinity stress.150 and 200 mmol/L NaCl treatment significantly decreased the activities of superoxide dismutase(SOD).Peroxidase(POD)firstly increased and then decreased with the increasing of the concentration of acetic acid.The rate of various antioxidant enzymes differed greatly in different salt concentration.Lower concentration NaCl solution tends to encourage total root growth,total project area and total surface area and the combination of more roots and more water tends to improve uptake of nutrients.Root system of wheat seedlings was comprised of fine roots with diameter between 0.15 mm and 0.30 mm.High concentration of NaCl inhibited growth of total root growth and total surface area.Root system of wheat seedlings was comprised of fine roots with diameter between 0.30 mm and 0.45 mm under high salt concentration.
作者
黄玲
杨文平
刘虎彪
周猛
梅沛沛
姚素梅
HUANG Ling;YANG Wenping;LIU Hubiao;ZHOU Meng;MEI Peipei;YAO Sumei(College of Life Science and Technology,Henan Institute of Science and Technology,Xinxiang 453003,China;Collaborative Innovation Center of Modern Biological Breeding of Henan Province,Xinxiang 453003,China)
出处
《河南科技学院学报（自然科学版）》
2020年第6期1-10,共10页
Journal of Henan Institute of Science and Technology(Natural Science Edition)
基金
国家自然科学基金(51509085)
河南省科技攻关项目(202102110165)
河南省高等学校重点科研项目(18B210003)
大学生创新创业项目(2019CX002)。
关键词
小麦
苗期
盐胁迫
形态指标
生理指标
wheat
seedling stage
salt stress
morphological index
physiological index
