摘要
专性寄生植物锁阳以干燥肉质茎入药,为常用中药材。在锁阳的生活史中,需要经历种子萌发、芽管状器管伸长、初生吸器形成、次生吸器形成等过程,其中种子萌发和初生吸器形成是锁阳完成生活史的最基本条件。目前,触发锁阳种子萌发、初生吸器形成的植物生长调节物质的阈值和种类并不清楚,导致人工调控锁阳生活史困难及栽培收益不高。本论文运用组织培养方法,研究赤霉素、生长素和细胞分类素等多种激素交互作用对锁阳种子萌发和吸器形成的影响。研究结果显示:(1)多种激素的共同作用促进锁阳球形原胚的发育。(2)B5培养基添加1.0 mg·L^-12,4-D,0.5 mg·L^-1 KT,1.0 mg·L^-1 GA3可以高效诱导锁阳种子愈伤组织形成,愈伤诱导率达13.7%±3.1%。(3)B5培养基添加0.5 mg·L^-12,4-D,0.25 mg·L^-1 KT可以高效诱导锁阳愈伤组织分化初生吸器,一些初生吸器继续分化成芽管状气管,延伸3~4 cm后,顶端膨大,形成新的初生吸器。本论文研究结果可为进一步研究锁阳种子萌发、初生吸器形成的内源激素变化规律及发生机制研究奠定基础。
Cynomorium songaricum Rupr.is an obligate parasitic plant widely used as traditional Chinese medicine and Mongolian medicine.Here,we firstly describe protocols for in vitro germination,callus induction and haustorium organogenesis in C.songaricum Rupr.In this study,adequate concentrations of gibberellic acid(GA3),in combination with other plant growth regulators in the medium,promoted embryo development and germination of C.songaricum seeds.The highest callus induction rate from seeds(13.7%)after a 40 d incubation was obtained with B5 medium adding 2,4-dichlorophenoxyacetic acid(2,4-D;1.0 mg·L^-1),kinetin(KT;0.5 mg·L^-1)and GA3(1.0 mg·L^-1).This resulted in callusformation in 13.7%±3.1%of seeds.Addition of 2,4-D(0.5 mg·L^-1)and KT(0.25 mg·L^-1)yielded highest haustorium organogenesis from calluses.Some primary haustorium branched to form adventitious roots of 3-4 cm in length.Subsequently,the tip of each adventitious root formed a nascent primary haustorium,which was then branched out into adventitious roots.The role of auxins(2,4-D in this study)in the formation of primary haustorium and adventitious roots from seed callus in C.songaricum was also discussed.
作者
岳鑫
陈贵林
YUE Xin;CHEN Gui-Lin(School of Life Science,Inner Mongolia University,Hohhot 010021;College of Pharmacy,Inner Mongolia Medical University,Hohhot 010021)
出处
《植物研究》
CAS
CSCD
北大核心
2020年第6期846-854,共9页
Bulletin of Botanical Research
基金
内蒙古自治区自然科学基金博士基金项目(2016BS0811)
内蒙古自治区科技创新引导奖励资金项目(2015cztxyd02)
教育部高等学校博士点基金(20091501110002)
内蒙古科技成果转化项目(CGZH2018127)。
