摘要
花青素合成酶(ANS)是花青素合成途经中的关键酶,可催化无色花色素转变成有色花色素。本文从NCBI数据库中获取铁皮石斛花青素合成酶基因(DoANS)的cDNA序列,并利用生物信息学方法对该基因进行了分析。分析结果表明:该基因完整的开放阅读框长1077 bp,共编码358个氨基酸,其中亮氨酸含量最高,占氨基酸总数的10.9%,半胱氨酸含量最低,占氨基酸总数的1.1%。理化性质分析表明,DoANS蛋白分子质量为39.59 kD,属于亲水性蛋白,理论等电点为5.42,无信号肽,主要分布于细胞质中;二级结构中无规则卷曲占比59.78%,延伸链占比15.08%,同时蛋白三级结构分析结果与之相符。最后,系统进化分析显示,DoANS与大花惠兰ANS的亲缘关系最近。
Anthocyanin synthase(ANS)is a key enzyme in the synthesis pathway of anthocyanidin,which can catalyze the conversion of colorless anthocyanidin to colored anthocyanidin.In this paper,the cDNA sequence of Dendrobium officinale ANS(DoANS)was obtained from NCBI database and analyzed by bioinformatics methods.Analysis results showed that its complete open reading frame was 1077 bp,encoding 358 amino acids,of which leucine content was the highest,accounting for 10.9%of the total amino acids,while cysteine content was the lowest,accounting for 1.1%of the total amino acids.Meanwhile,physicochemical analysis indicated that the molecular weight of DoANS was 39.59 kD,belonging to hydrophilic protein,with theoretical isoelectric point of 5.42.Then further analysis revealed that DoANS had no signal peptide and mainly distributed in the cytoplasm.Subsequently,secondary structure analysis showed that random coil accounted for 59.78%,while extended chain accounted for 15.08%and these results was consistent with the results of tertiary structure analysis.At last,phylogenetic analysis showed that DoANS was closely related to the ANS of Cymbidium hybrid.
作者
周娜娜
冯猜
徐僡
孙威
ZHOU Nana;FENG Cai;XU Hui;SUN Wei(Key Laboratory of Plant Physiology and Development Regulation/School of Life Science,Guizhou Normal University,Guiyang,Guizhou 550025,China;Key Iaboratory of State Forestry Administration on Biodiversity Conservation in Karst Mount ain Area of Southwest of China,Guiyang,Guizhou 550025,China)
出处
《贵州师范大学学报（自然科学版）》
CAS
2021年第1期36-40,94,共6页
Journal of Guizhou Normal University：Natural Sciences
基金
贵州师范大学资助博士科研项目(0516006)
贵州省重大科技专项(黔科合重大专项字[2019]3001-2)。
关键词
铁皮石斛
花青素合成酶基因
生物信息学
Dendrobium officinale
anthocyanin synthase gene
bioinformatics
