摘要
生育酚是水稻生长的必需物质,缺失会导致水稻植株生长迟缓。然而生育酚调控水稻生长的机理还很不明确。本研究通过对水稻生育酚缺失突变体sgd1-2的矮化成因进行分析,为阐明生育酚调控水稻生长的机理提供新的理论参考。测定Nip与sgd1-2中的细胞膜通透性和总脂肪酸组份。检测正常生长条件下Nip与sgd1-2中(dehydration-responsive element binding protein1, DREB1)类基因的表达量及游离脯氨酸含量。进行α淀粉酶活性分析和GA3处理,检测Nip和sgd1-2中SLENDER1 (SLR1)蛋白的积累情况,并分析GA1含量和SLR1基因的表达量。结果显示,sgd1-2的细胞膜通透性显著高于Nip,但二者之间总脂肪酸组份没有差异。在正常生长条件下sgd1-2中DREB1基因的表达量和游离脯氨酸含量明显高于Nip。sgd1-2对赤霉素(GA3)处理不敏感,同时sgd1-2中SLR1蛋白出现积累。Nip和sgd1-2中GA1的含量没有显著差异,sgd1-2中SLR1基因的表达量出现明显上调。上述结果证实生育酚缺失导致sgd1-2中细胞膜出现损伤,导致异位胁迫响应,同时诱导SLR1蛋白积累从而抑制水稻生长。
Tocopherols are essential components of the human diet having indispensable roles in living organisms.Tocopherols have been proved to be essential for rice development. However, the molecular mechanism underlying the relationship between tocopherol deficiency and rice development remained to be determined. To study how tocopherols function in rice development, the molecular mechanism underlying the development of sgd1-2 was analyzed. These results provided new insights into tocopherol functions in rice development. In this study, the cell-membrane penetrability and the fatty acid composition of Nip and sgd1-2 were analyzed. The expression levels of dehydration-responsive element binding protein1(DREB1) genes and the free proline content in sgd1-2 were determined under the optimal growth condition. The activity of α-amylase was analyzed. The Nip and sgd1-2 were treated with various concentrations of GA3. The accumulation of SLRENDER1(SLR1) protein was determined by western blot. The content of GA1 was determined. Cell-membrane penetrability in sgd1-2 was significant higher than Nip under the optimal growth condition. The total fatty acid composition of Nip and sgd1-2 was indistinguishable. The expression levels of DREB1 genes and the free proline content in sgd1-2 were also higher than those in Nip. sgd1-2 seedlings were less sensitive to exogenous GA3 than the Nip. The SLR1 protein was accumulated in sgd1-2. The GA1 levels of Nip and sgd1-2 was indistinguishable. The expression level of SLR1 was significantly up-regulated in sgd1-2 mutant than Nip. Our study confirms tocopherol deficiency leads to severely membrane damage in sgd1-2. And tocopherol deficiency induces ectopic stress response and restrains growth via enhancing SLR1 protein accumulation through increasing the transcript level.
作者
王迪
王建
程保山
李刚
徐卫军
袁彩勇
Wang Di;Wang Jian;Cheng Baoshan;Li Gang;Xu Weijun;Yuan Caiyong(Huaiyin Institute of Agricultural Sciences in Xuhuai Region of Jiangsu,Huai'an,223300;Jiangsu Key Laboratory for Eco-Agricultural Biotechnolo-gyaround Hongze Lake Huaiyin Normal University,Huaian,223300)
出处
《分子植物育种》
CAS
CSCD
北大核心
2020年第24期8247-8254,共8页
Molecular Plant Breeding
基金
淮安市自然科学研究计划(HAB201719)
江苏省环洪泽湖生态农业生物技术重点实验室自主研发课题项目(17HZHL009)共同资助。
关键词
生育酚
水稻
异位胁迫响应
赤霉素
Tocopherol
Rice
Ectopic stress response
Gibberellin
