摘要
为探究三江源地区典型高寒草地生态系统中丛枝菌根(AM)真菌群落变化的关键驱动过程,以三江源国家公园高寒荒漠、高寒草原、高寒草甸和高寒湿地4种典型草地生态系统为研究对象,基于Illumina-Miseq高通量测序手段,通过分析不同生态系统中AM真菌群落的物种及谱系组成,并结合群落谱系分析方法,探明影响AM真菌群落组成的各种因素,推断出AM真菌群落的构建机制及关键生态过程。结果表明:1)4种草地类型AM真菌多样性和群落结构存在明显差异,高寒荒漠、高寒草甸、高寒湿地的优势属均为球囊霉属(Glomus),高寒草原的优势属则为多样孢囊霉属(Diversispora)。2)高寒湿地AM真菌的OTU丰富度和谱系多样性指数均显著低于其他3种草地类型。3)植物群落组成、土壤含水量和有效N∶P是影响AM真菌群落组成的主要因子,其中土壤含水量是AM真菌群落物种组成的首要决定因素,而植物群落组成是决定AM真菌群落谱系组成的关键因子。4)4种草地类型的AM真菌群落谱系结构均为聚集模式,在高寒荒漠,AM真菌群落构建由随机过程决定,而在高寒草原、高寒草甸和高寒湿地,AM真菌群落受随机过程以及微弱的环境选择作用主导。
This study investigated the key processes driving arbuscular mycorrhizal(AM)fungal community changes in typical alpine grassland ecosystems,by systematically measuring AM fungal diversity and community composition in four typical grassland ecosystems of Sanjiangyuan National Park,based on the Illumina-Miseq high-throughput sequencing method.The ecosystems evaluated were:Alpine desert,alpine steppe,alpine meadow and alpine wetland.Ecological processes structuring the fungal communities were inferred according to phylogenetic patterns and species abundance distributions in different ecosystems.It was found that:1)The dominant genus in alpine desert,alpine meadow and alpine wetland was Glomus,while the dominant genus in alpine steppe was Diversispora.2)The OTU richness and phylogenetic diversity index of AM fungi in alpine wetland were significantly lower than those of the other three grassland ecosystem types.3)There were significant differences in species composition and phylogenetic composition of AM fungal communities among different grassland types.Plant community composition,soil water content,and available N∶P were the main factors affecting the composition of the AM fungal community.Soil water content was the primary determinant of the species composition of the AM fungal community,and plant community composition was a key factor in determining the phylogenetic composition of the AM fungal community.4)AM fungal communities were all phylogenetically clustered in the four grassland types.The phylogenetic structure of AM fungal communities in the different grassland types are clustered.In alpine deserts,AM fungal community assembly is determined by stochastic processes,while in alpine grasslands,alpine meadows and alpine wetlands,AM fungal community assemblage are determined by stochastic processes and weak responses to environmental filtering.
作者
李聪聪
周亚星
谷强
杨明新
朱传鲁
彭子原
薛凯
赵新全
王艳芬
纪宝明
张静
LI Cong-cong;ZHOU Ya-xing;GU Qiang;YANG Ming-xin;ZHU Chuan-lu;PENG Zi-yuan;XUE Kai;ZHAO Xin-quan;WANG Yan-fen;JI Bao-ming;ZHANG Jing(School of Grassland Science,Beijing Forestry University,Beijing 100083,China;Xining Natural Resources Comprehensive Sur-vey Center(The Former No.6 Gold Geological Party of PAP),China Geological Survey,Xining 810021,China;College of Life Science,University of Chinese Academy of Sciences,Beijing 100049,China;College of Resources and Environment,University of Chinese Academy of Sciences,Beijing 100049,China;Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xini-ng 810008,China)
出处
《草业学报》
CSCD
北大核心
2021年第1期46-58,共13页
Acta Prataculturae Sinica
基金
科技部重点研发项目(2016YFC0501802)
国家自然科学基金青年基金项目(31800380)
第二次青藏高原综合科学考察研究项目(2019QZKK0304)资助。
关键词
高寒草地生态系统
丛枝菌根真菌
谱系结构
群落构建
alpine grassland ecosystem
arbuscular mycorrhizal(AM)fungi
phylogenetic structure
community assembly
