摘要
目的:探讨产超广谱 β-内酰胺酶(ESBLs)大肠埃希菌检出率、基因型分布及其耐药性.方法:选取2018年1月-2020年1月医院细菌室分离出的82株大肠埃希菌,分析产ESBLs大肠埃希菌检出率、基因型分布情况以及耐药性.结果:82株大肠埃希菌中产ESBLs大肠埃希菌检出率为53.66%(44/82),其中43株(97.73%)为BlaCTX-M基因型,其中以BlaCTX-M-9为主,其余1株携带BlaTEM基因型,菌株为BlaTEM-1型.BlaCTX-M-9、BlaCTX-M-1对头孢曲松、头孢唑林、头孢噻肟耐药率为100%,对左氧氟沙星耐药率分别为93.10%、100%,对亚胺培南的耐药率为0;BlaCTX-M-1对头孢吡肟、氨曲南、头孢他啶耐药率为91.67%、100%、91.67%,均高于BlaCTX-M-9的37.93%、58.62%、37.93%,差异均有统计学意义(P<0.05).结论:大肠埃希菌中产ESBLs大肠埃希菌检出率较高,其中最常见分型为BlaCTX-M,并以BlaCTX-M-9型为主,其次为BlaCTX-M-1,两者对亚胺培南均不敏感,对头孢唑林、头孢曲松、头孢噻肟耐药、左氧氟沙星高度耐药,临床需根据药敏实验结果合理选择抗菌药物.
Objective:To investigate the detection rate,genotype distribution and drug resistance of Escherichia coli producing extended-spectrumβ-lactamases(ESBLs).Method:A total of 82 strains of Escherichia coli isolated from the hospital’s bacteria room from January 2018 to January 2020 were selected,the detection rate,genotype distribution and drug resistance of ESBLs-producing Escherichia coli were analyzed.Result:The detection rate of 82 strains of ESBLs was 53.66%(44/82),among which 43 strains(97.73%)were BlaCTX-M genotype,most of which were BlaCTX-M-9,and the remaining 1 strain carried BlaTEM genotype and strain was BlaTEM-1.The drug resistance rates of BlaCTX-M-9 and BlaCTX-M-1 to Ceftriaxone,Cefazolin and Cefotaxime were 100%,93.10%and 100%respectively to Levofloxacin,and 0 to Imipenem.The drug resistance rates of BlaCTX-M-1 against Cefepime,Amitracin and Ceftazidine were 91.67%,100%and 91.67%,higher than 37.93%,58.62%and 37.93%of BlaCTX-M-9,with statistically significant differences(P<0.05).Conclusion:The detection rate of ESBLs producing Escherichia coli is higher,among which BlaCTX-M is the most common type,and BlaCTX-M-9 is the main type,followed by BlaCTX-M-1.Both of them are not sensitive to Imipenem,but highly resistant to Cefazolin,Ceftriaxone,Cefotaxime and Levofloxacin.It is necessary to select antibiotics reasonably according to the results of drug sensitivity test.
作者
周鹰豪
蔡志军
刘军
ZHOU Yinghao;CAI Zhijun;LIU Jun(The Second Affiliated Hospital of Guangdong Medical University,Zhanjiang 524000,China;不详)
出处
《中国医学创新》
CAS
2020年第36期156-160,共5页
Medical Innovation of China
基金
广东省自然科学基金项目(2018A0303070018)。
关键词
大肠埃希菌
产超广谱β-内酞胺酶
基因型
耐药性
Escherichia coli
Production of extended-spectrumβ-lactamases
Genotype
Drug resistance
