摘要
研究利用高通量测序技术对感染球孢白僵菌高毒力菌株与野生型菌株72 h的大蜡螟4龄幼虫进行转录组分析,共检测到12770个共表达的基因。用|FC|≥4,Q≤0.001作为筛选标准,得到了的224个上调基因和227个下调基因。转录组分析结果表明,高毒力菌株侵染3 d后,大蜡螟体内脂肪酸合成,AMPK信号通路,糖原异生等代谢过程以及催化活性,RNA聚合等活性受到了高度抑制。除此之外,大蜡螟幼虫中的蛋白质的消化和吸收进程,肾素血管紧张素系统以及免疫反应被高度激活。本研究通过对侵染高毒力菌株的大蜡螟幼虫转录组进行分析,从转录组层面对宿主和高毒力病原体的相互作用进行了进一步研究。为筛选高毒力病原体提供了潜在靶点。
In this study,high-throughput sequencing technology was used to analyze the transcriptome of 4th instar larvae of Galleria mellonella that were infected with the highly virulent and wild-type Beauveria bassiana strains for 72 hours.A total of 12770 co-expressed genes were detected.By Using|FC|≥4,Q≤0.001 as the screening criteria,224 up-regulated genes and 227 down-regulated genes were obtained.Transcriptome analysis results showed that after 3 days of infection by the highly virulent strains,fatty acid synthesis,AMPK signaling pathway,glyconeogenesis,catalytic activity,RNA polymerization and other activities in the G.mellonella were highly inhibited.In addition,the process of digestion and absorption of proteins,the renin angiotensin system and the immune response were highly activated.This study analyzed the transcriptome of G.mellonella larvae infecting highly virulent strains,and further studied the interaction between the host and highly virulent pathogens from the transcriptome level.It provides a potential target for screening highly virulent pathogens.
作者
张力
李敬龙
ZHANG Li;LI Jing-long(College of Bioengineering,Qilu University of Technology(Shandong Academy of Sciences),State Key Laboratory of Bio-Based Materials and Green Papermaking,Jinan 250353,China)
出处
《齐鲁工业大学学报》
2021年第1期6-11,共6页
Journal of Qilu University of Technology
基金
山东省重点研发计划项目(2018GSF120014)。
关键词
白僵菌
大蜡螟
转录组分析
Galleria mellonella
Beauveria bassiana
transcriptome
