摘要
为有效利用藏橐吾Ligularia rumicifolia(Drumm.)S.W.Liu资源,以其子叶为外植体进行离体培养研究.结果发现,诱导愈伤组织的适宜培养基为MS+TDZ 1.0 mg/L+NAA 1.0 mg/L,诱导愈伤组织产生不定芽的适宜培养基为MS+6-BA 2.0 mg/L+NAA 0.5 mg/L,诱导率为69.56%.诱导子叶产生不定芽的适宜培养基为MS+6-BA 3.0 mg/L+NAA 0.8 mg/L,诱导率为72.33%.不定芽在1/2 MS+IAA 0.5 mg/L的培养基中生根率为70.00%.
For utilization of Ligularia rumicifolia(Drumm.)S.W.Liu,tissue culture in vitro was tested using cotyledon as explants.The results showed that the MS medium containing TDZ 1.0 mg/L and NAA 1.0 mg/L was suitable for callus induction.The maximum induction rate of adventitious shoots from callus was 69.56%when NAA(0.5 mg/L)and 6-BA(2.0 mg/L)were added in the MS medium.The MS medium containing 6-BA 3.0 mg/L and NAA 0.8 mg/L gave the better response to adventitious shoots induction from cotyledon explants,and the induction rate reached up to 72.33%.About 70.00%of the adventitious shoots rooted on 1/2 MS containing 0.5 mg/L IAA.
作者
李依
陆旭
郭萌
卢娇
王俊丽
LI Yi;LU Xu;GUO Meng;LU Jiao;WANG Junli(College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China)
出处
《河北大学学报（自然科学版）》
CAS
北大核心
2021年第1期55-59,共5页
Journal of Hebei University(Natural Science Edition)
基金
国家重点研发计划支持项目(2018YFD0201300)
一流大学一流学科建设项目(Yldxxk201819)。
关键词
藏橐吾
愈伤组织
不定芽
植株再生
Ligularia rumicifolia(Drumm.)S.W.Liu
callus
adventitious shoots
plant regeneration
