摘要
目的建立消瘤藤高效液相色谱(HPLC)特征指纹图谱,结合化学计量学方法评价其质量。方法采用HPLC法,以10批不同产地消瘤藤药材为样品,以共有峰峰面积为变量,采用相似度评价结合聚类分析和主成分分析从不同的侧重角度对色谱数据进行分析。结果标定出了12个共有峰,其中3个特征峰被识别,10批样品相似度均大于0.9,聚类分析将其分成4类,7-羟基-8-甲氧基香豆素、7-羟基香豆素和茵芋苷对消瘤藤品质有明显的影响,可作为其质量评价指标。结论HPLC特征指纹图谱结合化学计量学方法适用于不同产地消瘤藤的药材质量评价和控制。
Objective To establish an HPLC fingerprint of Pileostegia tomentella and to evaluate its quality in combination with chemometric methods.Methods HPLC method is used,taking 10 batches of medicinal herbs from different origins as samples,using common peak area as a variable,using similarity evaluation combined with cluster analysis and principal component analysis to analyze chromatographic data from different perspectives.Results 12 common peaks are calibrated,and 3 characteristic peaks are identified.The similarity of 10 batches of samples are all greater than 0.9.Cluster analysis divide them into 4 categories,7-hydroxy-8-methoxycoumarin,7-hydroxycoumarin and inulin have a significant effect on the quality of Pileostegia tomentella,which can be used as the quality evaluation index.Conclusion HPLC characteristic fingerprint combined with chemometrics method is suitable for the quality evaluation and control of medicinal materials of Pileostegia tomentella from different places.
作者
卢森华
黎强
樊文研
陈宇
刘吉成
LU Sen-hua;LI Qiang;FAN Wen-yan;CHEN Yu;LIU Ji-cheng(Yulin Center for Food and Drug Control,Yulin 537000,China;Nanning Food and Drug Inspection Institute,Nanning 530001,China)
出处
《现代中药研究与实践》
CAS
2020年第6期52-56,共5页
Research and Practice on Chinese Medicines
基金
广西食品药品安全科研项目(桂食药监科评〔2016〕8号)
玉林市科学研究与技术开发计划项目(玉市科201934037)。
