摘要
炭疽菌(Colletotrichum siamense)是橡胶树等多种热带作物炭疽病的主要病原。双组分系统(two component system)在病原真菌中参与菌体对外界环境变化的适应、耐药性和毒力的调控。该系统包括感受器和应答调节蛋白,SSK1(SLF-interacting SKP1-like1)是双组分系统中的应答调节蛋白。为了了解炭疽菌中SSK1互作蛋白,本研究克隆获得橡胶树炭疽菌的一个应答调节蛋白编码基因CsSSK1,大小为2750 bp,cDNA大小为2190 bp,编码729个氨基酸,包含1个内含子,具有1个cheY同系物接收结构域和10个复杂结构。并利用酵母双杂交技术,在橡胶树炭疽菌cDNA文库中对CsSSK1互作蛋白进行筛选,共筛选到10个互作蛋白,分别为转酮醇酶、金属内酰胺酶、锌乙醇脱氢酶、泛素亚型X1、微管蛋白以及5个未知或假定蛋白。该结果为进一步研究CsSSK1的功能、互作蛋白及调节机制提供基础。
Colletotrichum siamense is the main pathogen of anthracnose of rubber tree or other tropical crops.A two component system is involved in the adaptation of fungi to external environmental changes,drug resistance and virulence in pathogenic fungi.In pathogenic fungi,it includes receptors and SSK1(SLF-interacting SKP1-like1)response regulatory protein components.To obtain proteins interacting with SSK1 in C.siamense,a homologue gene CsSSK1,2750 bp long with cDNA 2190 bp,was cloned.It contained a cheY homologs receiving domains and 10 complex structures.Then the pGBKT7-CsSSK1 vector was constructed.By an yeast two hybrid system,ten proteins,including transketolase,β-lactamase,alcohol dehydrogenase,ubiquitin,tubulin and 5 unknown or hypothetical proteins,maight interacting with CsSSK1,were identified after sequencing and bioinformatics analysis.This result would provide a basis for further study of the functions,interaction proteins and regulatory mechanism of CsSSK1.
作者
方思齐
李泽栋
王记圆
何其光
李潇
刘文波
张宇
林春花
缪卫国
FANG Siqi;LI Zedong;WANG Jiyuan;HE Qiguang;LI Xiao;LIU Wenbo;ZHANG Yu;LIN Chunhua;MIAO Weiguo(College of Plant Protection,Hainan University/Key Laboratory of Green Forest Control and Prevention,Ministry of Education,Haikou,Hainan 570228,China)
出处
《热带作物学报》
CSCD
北大核心
2021年第1期198-204,共7页
Chinese Journal of Tropical Crops
基金
国家自然科学基金项目(No.31760499,No.31560495)
现代农业产业技术体系建设专项资金项目(No.CARS-33-BC1)。
关键词
橡胶树炭疽菌
双组分系统
应答调节蛋白SSK1
互作蛋白
Colletotrichum siamense
two component system
response regulatory protein SSK1
interaction protein
