摘要
目的:分析急性分泌性中耳炎(OME)患者外周血T淋巴细胞亚群与中耳积液炎症因子的相关性。方法:选取122例急性OME患者为病例组,同期100名接受健康体检者为对照组。检测所有人员外周血CD4^(+)T、CD8^(+)T淋巴细胞的含量、CD4^(+)/CD8^(+)T淋巴细胞比值及血清白细胞介素(IL)-2、IL-4、IL-6、IL-10及肿瘤坏死因子(TNF)-α水平的水平。病例组患者经耳内镜行鼓膜穿刺,分析耳积液中上述炎症因子的水平,并评估其与外周血T淋巴细胞亚群指标的相关性。结果:病例组CD4^(+)T淋巴细胞的含量及CD4^(+)/CD8^(+)T淋巴细胞比值均低于对照组(P<0.05),但CD8^(+)T淋巴细胞的含量高于对照组(P<0.05)。两组研究对象的血清炎症因子水平比较,差异均无统计学意义(P>0.05)。病例组耳积液中的炎症因子水平均高于血清(P<0.05),其中,IL-2、IL-4、IL-6、TNF-α等炎症因子水平与外周血CD4^(+)T淋巴细胞含量、CD4^(+)/CD8^(+)T淋巴细胞比值均呈负相关(P<0.05),但与CD8^(+)T淋巴细胞含量呈正相关(P<0.05)。此外,病例组患者耳积液中的IL-10水平也正相关于外周血CD4^(+)T淋巴细胞含量及CD4^(+)/CD8^(+)T淋巴细胞比值(P<0.05),但其与CD8^(+)T淋巴细胞含量呈负相关(P<0.05)。结论:急性OME患者耳积液中的炎症因子水平高于血清,且与外周血T淋巴细胞亚群指标相关。
Objective:To analyze the correlations between T lymphocyte subsets in peripheral blood and inflammatory factors in middle ear effusion in patients bearing acute otitis media with effusion(OME).Methods:122 patients with acute OME were selected as the case group,and 100 healthy people were selected as the control group.Their percentage of CD4^(+)T lymphocyte and CD8^(+)T lymphocyte,the ratio of CD4^(+)/CD8^(+)T lymphocyte in the peripheral blood,and the interleukin(IL)-2,IL-4,IL-6,IL-10 and tumor necrosis factor(TNF)-αin serum were detected.The patients in the case group were punctured with tympanic membrane under otoscope,the levels of inflammatory factors in middle ear effusion were analyzed and their correlations with T lymphocyte subgroups in peripheral blood were evaluated.Results:The percentage of CD4^(+)T lymphocyte and the ratio of CD4^(+)/CD8^(+)T lymphocyte in the case group were significantly lower than those in the control group(P<0.05),while their percentage of CD8^(+)T lymphocyte was notable higher than the control group(P<0.05).There was no significant difference in the levels of serum inflammatory factors between the subjects in the two groups(P>0.05).The levels of inflammatory factors in middle ear effusion of the patients in the case group were obviously higher than those in serum(P<0.05),among them,the levels of IL-2,IL-4,IL-6 and TNF-αwere negatively correlated with the percentage of CD4^(+)T lymphocyte and the ratio of CD4^(+)/CD8^(+)T lymphocyte(P<0.05),but positively related to the percentage of CD8^(+)T lymphocyte(P<0.05).The level of IL-10 was positively associated with the percentage of CD4^(+)T lymphocyte and the ratio of CD4^(+)/CD8^(+)T lymphocyte(P<0.05),while it was negatively correlated with the percentage of CD8^(+)T lymphocyte(P<0.05).Conclusion:The levels of inflammatory factors in ear effusion are significantly higher than those in serum and are correlated with the T lymphocyte subgroups indicators in peripheral blood.
作者
崔喜财
赵恒威
张鲁壮
宋晓洁
崔凯
CUI Xi-cai;ZHAO Heng-wei;ZHANG Lu-zhuang;SONG Xiao-jie;CUI Kai(Department of Laboratory Medicine,Fourth Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110101,Liaoning;Department of General Surgery,Qinhuangdao Workers'Hospital,Qinhuangdao 066200,Hebei,China)
出处
《川北医学院学报》
CAS
2021年第1期68-71,共4页
Journal of North Sichuan Medical College
基金
河北省秦皇岛市科学技术研究与发展计划(201703A039)。
关键词
急性分泌性中耳炎
外周血T淋巴细胞亚群
中耳积液
炎症因子
Acute otitis media with effusion
T lymphocyte subgroups in peripheral blood
Middle ear effusion
Inflammatory factors
