摘要
目的建立高效液相色谱法(HPLC)测定不同产地白花蛇舌草中去乙酰车叶草酸甲酯的含量,为改善白花蛇舌草的质量控制提供参考。方法采用Waters SymmetryShield RP C_(18)(4.6 mm×250 mm,5μm)色谱柱;流动相:乙腈-水(4:96);检测波长:236 nm;柱温:25℃;流速:1.0 ml/min;进样量:10μl。结果去乙酰车叶草酸甲酯在13.4~335.0μg/ml范围内线性关系良好;平均回收率为99.37%,RSD为0.3%。结论本方法简便、重复性好、准确度高,可用于白花蛇舌草的质量控制。
Objective To establish an HPLC method for the determination of deacetyl asperulosidic acid methyl ester in Hedyotis diffusa Willd from different regions and to provide a reference for quality control of Hedyotis diffusa.Methods A Waters SymmetryShield RP C18column(4.6 mm×250 mm,5μm)was adopted.The mobile phase was acetonitrile-water(4:96)with detection wavelength set at 236 nm,the column temperature was 25℃and the flow rate was 1.0 ml/min.Results The linear range of deacetyl asperulosidic acid methyl ester was 13.4-335.0μg/ml.The average recovery of oleanolic acid was 99.37%,with RSD of 0.3%.Conclusion The method is simple,reproducible and accurate,and can be applied to the quality control of Hedyotis diffusa.
作者
丁建兰
黄宁
赵超
袁丽
王毅梅
DING Jian-lan;HUANG Ning;ZHAO Chao;YUAN Li;WANG Yi-mei(Yingtan Comprehensive Inspection and Testing Center,Yingtan 335000,China)
出处
《食品与药品》
CAS
2021年第1期55-57,共3页
Food and Drug
